Gene

Thermus aquaticus

Molecular biology

Multiplex polymerase chain reaction

Biology

Sequencing by ligation

DNA extraction

Hot start PCR

Base sequence

DNA clamp

Genetics

DNA nanoball sequencing

Organic chemistry

Genomic library

Primer dimer

Polymerase

DNA polymerase I

Reverse transcriptase

DNA polymerase II

Multiple displacement amplification

Chemistry

Primer (cosmetics)

Taq polymerase

Polymerase chain reaction

DNA polymerase

DNA sequence

The ΔTaqr` DNA polymerase is a new, genetically modified version of standard Taq DNA polymerase which lacks the 5′ → 3′-exonuclease activity. The present study was designed to investigate the use of ΔTaqr` DNA polymerase for direct cycle sequencing. Results show that ΔTaqr` DNA polymerase can be used for direct cycle sequencing of the PCR amplified DNA, either from an asymmetrically amplified template (by PCR), double stranded DNA template, PCR amplified DNA cloned into a plasmid vector or from a single stranded template. The primer to template ratio and number of cycles necessary for best sequence data have been determined. From these results we conclude that ΔTaqr` DNA polymerase is a highly versatile enzyme which can be used for DNA sequence determinations by direct cycle sequencing.

Direct Cycle Sequencing with Δ<i>Taq</i><sup>r`</sup>DNA Polymerase

Direct Cycle Sequencing with ΔTaqr`DNA Polymerase

Direct Cycle Sequencing with ΔTaq^r`DNA Polymerase