International Journal of Systematic and Evolutionary Microbiology

A novel Gram-negative, aerobic, motile and rod-shaped bacterium was isolated from Qurugöl Lake located in a mountainous region near Tabriz city in the north-west of Iran. Growth occurred at pH 6–10 (optimum, pH 7±0.5) and at 10–40 °C (optimum, 30 °C). Strain RCRI4 T was able to grow in the absence and presence of NaCl to 3 % (w/v). The major fatty acids were C 17 : 0 , C 16 : 1 ω7 c /C 15 iso3-OH, C 17 : 1 ω8 c and C 16 : 0 . The G+C content of genomic DNA was 45.3 mol%. Based on the 16S rRNA and gyrB gene sequences, phylogenetic analyses indicated that strain RCRI4 T associated with the genus Alishewanella , and closely related type strains include Alishewanella agri BLO6 T (97.8 %), Alishewanella aestuarii B11 T (97.7 %), Rheinheimera perlucida BA131 T (97.5 %), Alishewanella fetalis CCUG 30811 T (97.3 %) and Alishewanella jeotgali MS1 T (97.1 %). The level of DNA–DNA relatedness between strain RCRI4 T and phylogenetically the closest related strains, A. agri BLO6 T and R. perlucida BA131 T , was 9 and 14 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic results, it is suggested that strain RCRI4 T represents a novel species of the genus Alishewanella , for which the name Alishewanella tabrizica sp. nov. is proposed. The type strain is RCRI4 T ( = LMG 26473 T = JCM 17275 T = KCTC 23723 T ).

Alishewanella tabrizica sp. nov., isolated from Qurugöl Lake