Selective stimulation in a natural community of methane oxidizing bacteria: Effects of copper on pmoA transcription and activity

Published on Oct 1, 2013in Soil Biology & Biochemistry5.795
· DOI :10.1016/J.SOILBIO.2013.05.027
Adrian Ho21
Estimated H-index: 21
(MPG: Max Planck Society),
Claudia Lüke24
Estimated H-index: 24
(MPG: Max Planck Society)
+ 1 AuthorsPeter Frenzel50
Estimated H-index: 50
(MPG: Max Planck Society)
Sources
Abstract
Abstract Copper is an essential element, but often not readily available to microorganisms potentially causing copper limitation in situ . In most methane oxidizing bacteria (methanotrophs), the copper-containing membrane bound methane monooxygenase (pMMO) catalyzes the first step in methane oxidation. We focused on the response of methanotrophic activity to a copper amendment in a rice paddy soil, measuring methane oxidation rates and following changes of pmoA diversity (a gene encoding for a subunit of the pMMO) both at DNA and mRNA level with a diagnostic microarray. Using transcripts (mRNA) as a proxy for taxon-specific activity, we found a clear separation between overall (DNA) and active (mRNA) community, the latter being dominated by type Ia methanotrophs. Copper addition transiently stimulated methane uptake. This stimulation correlated to a change within Methylosarcina -related transcripts shifting from Methylosarcina quisquiliarum to a yet uncultivated Methylosarcina sp. Overall, we demonstrate copper limitation in a soil, but to an extent that still allowed pmoA expression. The taxon-specific response suggests a fine-scale niche differentiation between methanotrophs, with Methylobacter and Methylosarcina reacting immediately to a copper amendment.
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