Quantitative analysis (chemistry)

High-performance liquid chromatography

Chromatography

Biochemistry

Antioxidant

Ammonium acetate

Chemistry

Ergothioneine

Detection limit

Hydrophilic interaction chromatography

Journal of Separation Science

A new hydrophilic interaction ultra‐performance LC method was established for the whole blood measurement of L‐ergothioneine. Chromatographic separation was achieved in a fairly short time, less than 4 min, on a 100 × 2.1 mm Acquity UPLC BEH HILIC 1.7 μm column with a mobile phase consisting of a mixture of 100 mmol/L ammonium acetate/ACN/water (5:85:10, v/v/v) that flowed isocratically at 0.250 mL/min. The LOD and the limit of quantification were 3.85 and 11.67 μmol/L, respectively. The method exhibited linearity in a concentration range of 15.63–1000 μmol/L ( R 2 &gt; 0.999). Mean recovery was 96.34% whereas intraassay and interassay precision were 1.52 and 1.82% RSD, respectively. On the whole, the developed method is simple, fast, precise, accurate, and sensitive and may be useful for routine analyses.

Quantification of L‐ergothioneine in whole blood by hydrophilic interaction ultra‐performance liquid chromatography and <scp>UV</scp>‐detection

Quantification of L‐ergothioneine in whole blood by hydrophilic interaction ultra‐performance liquid chromatography and UV‐detection