Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture
Volume: 22, Issue: 5, Pages: 939 - 946
Published: Jan 20, 2012
Improvements in technology have reduced the cost of DNA sequencing to the point that the limiting factor for many experiments is the time and reagent cost of sample preparation. We present an approach in which 192 sequencing libraries can be produced in a single day of technician time at a cost of about $15 per sample. These libraries are effective not only for low-pass whole-genome sequencing, but also for simultaneously enriching them in pools...