Epidermis (zoology)

Biotechnology

Biology

Materials science

Skin barrier

Absorption (acoustics)

Dermatology

Biochemistry

Human skin

Genetics

In vivo

Organic chemistry

Stratum corneum

Ex vivo

Toxicity

Anatomy

In vitro

Transepidermal water loss

Composite material

Medicine

Chemistry

Sulfur mustard

Histology

Pathology

Cutaneous and Ocular Toxicology

Previous studies in our laboratory have demonstrated that barrier creams, comprising perfluorinated polymers, are effective against the chemical warfare agent sulphur mustard (SM) when evaluated using human skin in vitro. The purpose of this follow-up study was to further evaluate three candidate (perfluorinated) barrier creams against SM (vapour) using the domestic white pig. The severity and progression of the resulting skin lesions were quantified daily for three weeks post-exposure using biophysical measurements of transepidermal water loss (TEWL) and skin reflectance spectroscopy (SRS). Skin biopsies obtained post-mortem were evaluated by light microscopy and additional skin samples were obtained from adjacent (unexposed) skin sites for a comparative in vitro skin absorption study. Samples of SM vapour within the dosing chambers were measured ex vivo to ascertain the exposure dose (Ct). The three creams were highly effective against SM in vivo (Ct approximately 5000 mg.min.m(-3)): After 3 weeks, barrier cream pre-treated sites were not significantly different from control (unexposed) skin when evaluated by TEWL, SRS or histology. In contrast, skin exposed to SM without pre-treatment showed evidence of persistent damage that was consistent with the slow healing time observed in humans. The amount of SM absorbed in vitro in untreated pig skin was similar to that required to cause comparable lesions in human skin (8-20 and 4-10 microg.cm(-2), respectively), further validating the use of pigs as a toxicologically-relevant dermal model for SM exposure.

Evaluation of Barrier Creams Against Sulphur Mustard: (II)<i>In Vivo</i>and<i>In Vitro</i>Studies using the Domestic White Pig

Evaluation of Barrier Creams Against Sulphur Mustard: (II)In VivoandIn VitroStudies using the Domestic White Pig