Gene

Function (biology)

Protein function prediction

World Wide Web

Biology

Protein tertiary structure

The Internet

Biochemistry

Data mining

Computer science

Protein structure

Computational biology

Genetics

Bioinformatics

Sequence (biology)

Protein function

UniProt

Structural genomics

Web server

Protein structure prediction

Sequence alignment

Peptide sequence

Threading (protein sequence)

Nature Protocols

The iterative threading assembly refinement (I-TASSER) server is an integrated platform for automated protein structure and function prediction based on the sequence-to-structure-to-function paradigm. Starting from an amino acid sequence, I-TASSER first generates three-dimensional (3D) atomic models from multiple threading alignments and iterative structural assembly simulations. The function of the protein is then inferred by structurally matching the 3D models with other known proteins. The output from a typical server run contains full-length secondary and tertiary structure predictions, and functional annotations on ligand-binding sites, Enzyme Commission numbers and Gene Ontology terms. An estimate of accuracy of the predictions is provided based on the confidence score of the modeling. This protocol provides new insights and guidelines for designing of online server systems for the state-of-the-art protein structure and function predictions. The server is available at http://zhanglab.ccmb.med.umich.edu/I-TASSER .

I-TASSER: a unified platform for automated protein structure and function prediction