Interpretation of protein quantitation using the Bradford assay: Comparison with two calculation models
Abstract
The Bradford assay is a simple method for protein quantitation, but variation in the results between proteins is a matter of concern. In this study, we compared and normalized quantitative values from two models for protein quantitation, where the residues in the protein that bind to anionic Coomassie Brilliant Blue G-250 comprise either Arg and Lys (Method 1, M1) or Arg, Lys, and His (Method 2, M2). Use of the M2 model yielded much more...
Paper Details
Title
Interpretation of protein quantitation using the Bradford assay: Comparison with two calculation models
Published Date
Mar 1, 2013
Journal
Volume
434
Issue
1
Pages
178 - 180
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