Gene

Molecular biology

Bacteria

Biology

Terminator (solar)

Expression vector

Amylase

Bacillales

Biochemistry

Genetics

Ionosphere

Physics

Bacillus subtilis

Bacillaceae

Astronomy

Antibiotics

Microbiology

Enzyme

Chemistry

Plasmid

Tetracycline

Gene expression

Recombinant DNA

Journal of Fermentation and Bioengineering

In order to construct a tetracycline (Tc) inducible expression-secretion vector system, the α-amylase structural gene of null Bacillus subtilis null strain 1A412 was isolated, and inserted between the promoter-operator and the terminator region of a Tc-resistant (Tc r ) gene. When the α-amylase gene was expressed in null B. subtilis null on a plasmid, the expression of the α-amylase gene could be modulated by the Tc concentration. α-Amylase activity was increased approximately 15-fold by adding 500 ng/ml Tc.

The tetracycline inducible expression of α-amylase in Bacillus subtilis