Biology

Biological activity

Nitric oxide

Biochemistry

Botany

Stereochemistry

Organic chemistry

Pharmacognosy

In vitro

Macrophage

Phenanthrene

Phenanthrenes

Chemistry

Dendrobium

Bioorganic & Medicinal Chemistry Letters

Bioactivity-guided isolation of the methanol extract of the stems of Dendrobium nobile yielded a new phenanthrene together with nine known phenanthrenes and three known bibenzyls. Their structures were elucidated by analysis of the spectroscopic data including 2D-NMR. All of the isolates were evaluated for their potential to inhibit the LPS-induced production of nitric oxide in murine macrophage RAW 264.7 cells. Compounds 1–4, 7–13 inhibited nitric oxide production with the IC50 values ranging from 9.6 μM to 35.7 μM.

Phenanthrenes from Dendrobium nobile and their inhibition of the LPS-induced production of nitric oxide in macrophage RAW 264.7 cells