Glucuronide

High-performance liquid chromatography

Pharmacokinetics

Chromatography

Column chromatography

Elution

Cardiology

Biochemistry

Phenprocoumon

Extraction (chemistry)

Warfarin

Urine

Pharmacology

Medicine

Chemistry

Atrial fibrillation

Journal of Chromatography B Biomedical Sciences and Applications

The determination of the anticoagulant phenprocoumon in plasma, after acidification and extraction with 1,2-dichloroethane was effected through isocratic high-performance liquid chromatography; a C18 reversed-phase column was used as stationary phase using aqueous acetonitrile as eluent and UV detection at 313 nm; p-chlorophenprocoumon was used as internal standard. A high proportion of phenprocoumon in urine is eliminated as the glucuronide and must be hydrolyzed enzymatically before extraction; the same column and detector as for plasma were used, but with gradient elution. The method was used in the range 0.1-5 mg/1, the sensitivity was 0.1 mg/1 for plasma and 0.02 mg/1 for urine, the precision was in the range 3-5% and the absence of interference due to other anticoagulants, drugs or endogenous compounds allows the specific determination of phenprocoumon in plasma and urine from patients and volunteers in clinical relevant cases, drug interaction, compliance, toxicological and pharmacokinetic studies.

Determination of the anticoagulant phenprocoumon in human plasma and urine by high-performance liquid chromatography