An updated method for the isolation and culture of primary calf hepatocytes

Volume: 191, Issue: 3, Pages: 323 - 326
Published: Mar 1, 2012
Abstract
Primary hepatocytes are commonly used during in vitro studies, but care must be taken with isolation and culture of the cells to ensure their viability. In this study, hepatocytes were isolated from the liver (caudate process) of a newborn calf by the collagenase perfusion and digestion method. The trypan blue exclusion method was used to determine total cell number and the survival rate of hepatocytes, while hepatocyte function was assessed by...
Paper Details
Title
An updated method for the isolation and culture of primary calf hepatocytes
Published Date
Mar 1, 2012
Volume
191
Issue
3
Pages
323 - 326
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