DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1

Published on Mar 4, 2015in BMC Plant Biology3.497
· DOI :10.1186/S12870-015-0453-1
Silvia Santopolo3
Estimated H-index: 3
,
Alessandra Boccaccini7
Estimated H-index: 7
+ 6 AuthorsPaola Vittorioso13
Estimated H-index: 13
Sources
Abstract
Background The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of the light-mediated seed germination process. DAG1 acts downstream PHYTOCHROME INTERACTING FACTOR3-LIKE 5 (PIL5), the master repressor, and negatively regulates gibberellin biosynthesis by directly repressing the biosynthetic gene AtGA3ox1. The Dof protein DOF AFFECTING GERMINATION (DAG2) shares a high degree of aminoacidic identity with DAG1. While DAG1 inactivation considerably increases the germination capability of seeds, the dag2 mutant has seeds with a germination potential substantially lower than the wild-type, indicating that these factors may play opposite roles in seed germination.
Figures & Tables
Download
📖 Papers frequently viewed together
123 Citations
16 Citations
19 Citations
References37
Newest
#2Silvia SantopoloH-Index: 3
Last. Paola VittoriosoH-Index: 13
view all 8 authors...
22 CitationsSource
#2Silvia SantopoloH-Index: 3
Last. Paola VittoriosoH-Index: 13
view all 9 authors...
Background The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of seed germination acting downstream of the master repressor PHYTOCROME INTERACTING FACTOR3-LIKE 5 (PIL5). Among others, PIL5 induces the expression of the genes encoding the two DELLA proteins GA INSENSITIVE 1 (GAI) and REPRESSOR OF ga1-3 (RGA).
19 CitationsSource
#1Hui Shi (PKU: Peking University)H-Index: 14
#2Shangwei Zhong (PKU: Peking University)H-Index: 15
Last. Xing Wang Deng (PKU: Peking University)H-Index: 131
view all 6 authors...
Seed germination is the first step for seed plants to initiate a new life cycle. Light plays a predominant role in promoting seed germination, where the initial phase is mediated by photoreceptor phytochrome B (phyB). Previous studies showed that PHYTOCHROME-INTERACTING FACTOR1 (PIF1) represses seed germination downstream of phyB. Here, we identify a positive regulator of phyB-dependent seed germination, LONG HYPOCOTYL IN FAR-RED1 (HFR1). HFR1 blocks PIF1 transcriptional activity by forming a he...
81 CitationsSource
#1Mélanie Noguero (INRA: Institut national de la recherche agronomique)H-Index: 5
#2Rana Muhammad Atif (INRA: Institut national de la recherche agronomique)H-Index: 10
Last. Richard D. Thompson (INRA: Institut national de la recherche agronomique)H-Index: 56
view all 4 authors...
a b s t r a c t The DOF (DNA-binding One Zinc Finger) family of transcription factors is involved in many fundamental processes in higher plants, including responses to light and phytohormones as well as roles in seed maturation and germination. DOF transcription factor genes are restricted in their distribution to plants, where they are in many copies in both gymnosperms and angiosperms and also present in lower plants such as the moss Physcomitrella patens and in the alga Chlamydomonas reinhar...
157 CitationsSource
#1Silvia Elizabeth Ibarra (UBA: University of Buenos Aires)H-Index: 6
#2Gabriela Alejandra Auge (UBA: University of Buenos Aires)H-Index: 11
Last. Javier Francisco Botto (UBA: University of Buenos Aires)H-Index: 24
view all 4 authors...
In Arabidopsis seeds, germination is promoted only by phytochromes, principally phytochrome B (phyB) and phytochrome A (phyA). Despite the abundant information concerning the molecular basis of phyB signaling downstream of PIF1/PIL5, the signaling network inducing germination by phyA is poorly known. Here, we describe the influence of phyA on the transcriptome of Arabidopsis seeds when germination is induced by a far-red (FR) pulse. The expression of 11% of the genome was significantly regulated...
23 CitationsSource
#1Sara Hernando-Amado (UPM: Technical University of Madrid)H-Index: 2
#2Virginia González-Calle (UPM: Technical University of Madrid)H-Index: 3
Last. Cristina Barrero-Sicilia (UPM: Technical University of Madrid)H-Index: 10
view all 4 authors...
Transcription factors (TFs) are proteins that have played a central role both in evolution and in domestication, and are major regulators of development in living organisms. Plant genome sequences reveal that approximately 7% of all genes encode putative TFs. The DOF (DNA binding with One Finger) TF family has been associated with vital processes exclusive to higher plants and to their close ancestors (algae, mosses and ferns). These are seed maturation and germination, light-mediated regulation...
45 CitationsSource
#1Paloma Rueda-Romero (UPM: Technical University of Madrid)H-Index: 3
#2Cristina Barrero-Sicilia (UPM: Technical University of Madrid)H-Index: 10
Last. Luis Oñate-Sánchez (UPM: Technical University of Madrid)H-Index: 18
view all 5 authors...
Seed dormancy prevents seeds from germinating under environmental conditions unfavourable for plant growth and development and constitutes an evolutionary advantage. Dry storage, also known as after-ripening, gradually decreases seed dormancy by mechanisms not well understood. An Arabidopsis thaliana DOF transcription factor gene (DOF6) affecting seed germination has been characterized. The transcript levels of this gene accumulate in dry seeds and decay gradually during after-ripening and also ...
86 CitationsSource
#1H. S. Kushwaha (Integral University)H-Index: 8
#2Shubhra Gupta (Deen Dayal Upadhyay Gorakhpur University)H-Index: 5
Last. Dinesh Yadav (Deen Dayal Upadhyay Gorakhpur University)H-Index: 17
view all 5 authors...
The Dof (DNA binding with One Finger) family represents a classic zinc-finger transcription factors involved with multifarious roles exclusively in plants. There exists great diversity in terms of number of Dof genes observed in different crops. In current study, a total of 28 putative Dof genes have been predicted in silico from the recently available whole genome shotgun sequence of Sorghum bicolor (L.) Moench (with assigned accession numbers TPA:BK006983–BK007006 and TPA:BK007079–BK007082). T...
73 CitationsSource
#1Jeongmoo Park (KAIST)H-Index: 12
#2Nayoung Lee (KAIST)H-Index: 9
Last. Giltsu Choi (KAIST)H-Index: 36
view all 5 authors...
A previous study showed that SOMNUS (SOM), which encodes a C3H-type zinc finger protein, is a key negative regulator of seed germination that acts downstream of PHYTOCHROME INTERACTING FACTOR3-LIKE5 (PIL5). However, it was not determined if PIL5 is the sole regulator of SOM expression. Public microarray data suggest that the expression of SOM mRNA is regulated also by ABSCISIC ACID INSENSITIVE3 (ABI3), another key regulator of seed germination. By analyzing abi3 mutants and ABI3 overexpression l...
91 CitationsSource
#1Stefano Gabriele (Sapienza University of Rome)H-Index: 1
#2Annalisa Rizza (Sapienza University of Rome)H-Index: 5
Last. Paola Vittorioso (Sapienza University of Rome)H-Index: 13
view all 6 authors...
*† SUMMARY We have previously shown that inactivation of the gene encoding the Arabidopsis thaliana transcription factor DOF AFFECTING GERMINATION 1 (DAG1) renders seed germination more sensitive to both phytochrome B (phyB) and gibberellins (GA). dag1 mutant seeds require less red (R) light fluence and a lower GA concentration than WT to germinate. Here, we show that inactivation of the gene PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 (PIL5) results in down-regulation of DAG1. Inactivation of PIL5 ...
100 CitationsSource
Cited By26
Newest
#1Shilin Sun (Shenyang Agricultural University)
Last. Huiyan Guo (Shenyang Agricultural University)
view all 0 authors...
Source
#1Hongying Yu (Fujian Agriculture and Forestry University)
#2Yaying Ma (Fujian Agriculture and Forestry University)H-Index: 2
Last. Ray Ming (UIUC: University of Illinois at Urbana–Champaign)H-Index: 62
view all 5 authors...
DNA-binding with one finger (Dof) are plant-specific transcription factors involved in numerous pathways of plant development, such as abiotic stresses responses. Although genome-wide analysis of Dof genes has been performed in many species, but these genes in spinach have not been analyzed yet. We performed a genome-wide analysis and characterization of Dof gene family in spinach (Spinacia oleracea L.). Twenty-two Dof genes were identified and classified into four groups with nine subgroups, wh...
Source
Long non-coding RNA (lncRNA) plays an important regulatory role in plant growth and development, but its systematic identification and analysis in Eucalyptus has not yet been reported. Cold stress has a huge impact on the survival and yield of Eucalyptus seedlings, but the regulatory mechanism of lncRNA in Eucalyptus in response to cold stress is still unclear. In this study, the transcriptomes of young leaves of Eucalyptus urophylla S.T.Blake under low-temperature treatment and restoration were...
Source
#1Neeta Lohani (University of Melbourne)H-Index: 3
#2Saeid Babaei (University of Melbourne)
Last. Prem L. Bhalla (University of Melbourne)H-Index: 36
view all 4 authors...
DNA binding with one finger (DOF) proteins are plant-specific transcription factors that play roles in diverse plant functions. However, little is known about the DOF protein repertoire of the allopolyploid crop, Brassica napus. This in silico study identified 117 Brassica napus Dof genes (BnaDofs) and classified them into nine groups (A, B1, B2, C1, C2.1, C2.2, C3, D1, and D2), based on phylogenetic analysis. Most members belonging to a particular group displayed conserved gene structural organ...
Source
#1Abirami Rajavel (GAU: University of Göttingen)H-Index: 1
#2Selina Klees (GAU: University of Göttingen)H-Index: 1
Last. Mehmet Gültas (GAU: University of Göttingen)H-Index: 7
view all 7 authors...
Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF-TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of Brassica napus L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high a...
Source
#1Ningmei Yang (HAU: Huazhong Agricultural University)H-Index: 3
#2Shuang Li (HAU: Huazhong Agricultural University)H-Index: 1
Last. Guangda Ding (HAU: Huazhong Agricultural University)H-Index: 17
view all 10 authors...
Oilseed rape (Brassica napus L.) has high nitrogen (N) requirement for its optimal growth and yield. However, little is known about the transcriptomic reprogramming of rapeseed in response to N deficiency. Two rapeseed genotypes with contrasting N use efficiency (NUE) were used to perform whole-transcriptome sequencing on the shoots and roots supplied with or without N in a time-course experiment. A total of 24,435 differentially expressed genes (DEGs) were identified. Among them, 8811 genes wer...
Source
#1Merve Seven (Yeditepe University)H-Index: 1
#2Hülya Akdemir (GIT: Gebze Institute of Technology)H-Index: 6
Seed germination is a multi-staged complex process during seed plant life cycle, and it is tightly regulated through a coordinated expression of diverse genes in diverse tissues. As regulatory molecules of gene expression, determination of transcription factors is crucial to understanding molecular basis and regulatory network of germination process and seedling establishment. However, limited data on the contributions of these transcription factors to the germination of crop barley (Hordeum vul...
2 CitationsSource
#1Siyan Qi (College of Horticulture)H-Index: 6
#2Wenchun Ma (College of Horticulture)H-Index: 2
Last. Libo Xing (College of Horticulture)H-Index: 12
view all 10 authors...
Plant-specific CDF genes encode a class of transcription factors which play crucial roles in plant flowering, especially in photoperiod-regulated flowering. But little is known about the potential ...
Source
#1Yue Wang (CAU: China Agricultural University)H-Index: 1
#2Donovan C. Bailey (NMSU: New Mexico State University)H-Index: 1
Last. Xuehui Dong (CAU: China Agricultural University)H-Index: 2
view all 4 authors...
This study was conducted to explore specific chill models and the mechanisms underlying rhizome bud dormancy break in Polygonatum kingianum. Rhizome buds were subjected to various chilling temperatures for different duration and then transferred to warm conditions for germination and subsequent evaluation of their response to temperature and chilling requirements. A CUkingianum model was constructed to describe the contribution of low temperature to the chill unit, and it was suggested that 2.97...
1 CitationsSource
#1An Yan (NTU: Nanyang Technological University)H-Index: 5
#2Zhong Chen (NTU: Nanyang Technological University)H-Index: 92
Seed dormancy and germination are two closely linked physiological traits that have great impacts on adaptation and survival of seed plants. Seed dormancy strengthen and germination potential are comprehensively influenced by a variety of internal factors and external environment cues. Environmental factors, such as water content, light condition, ambient temperature, and nitrogen availability, act as signal input to determine whether seeds keep in a dormant state or start to germinate. Light, t...
10 CitationsSource