The Anti-Wrinkle Efficacy of Argireline, a Synthetic Hexapeptide, in Chinese Subjects

Published on Feb 16, 2013in American Journal of Clinical Dermatology5.056
· DOI :10.1007/S40257-013-0009-9
Yuan Wang2
Estimated H-index: 2
(Xi'an Jiaotong University),
Mei Wang2
Estimated H-index: 2
(Xi'an Jiaotong University)
+ 3 AuthorsJia Huo7
Estimated H-index: 7
(Xi'an Jiaotong University)
Background Argireline is a synthetic peptide that is patterned from the N-terminal end of the protein SNAP-25 and has been shown to reduce the degree of facial wrinkles. It is reported to inhibit vesicle docking by preventing formation of the ternary SNARE complex and by interfering in catecholamine release. The anti-wrinkle efficacy of argireline has not been studied in Chinese subjects. Objective The objective of the study was to evaluate the safety and efficacy of argireline in the treatment of periorbital wrinkles in Chinese subjects. Methods A total of 60 subjects received a randomized treatment of argireline or placebo in a ratio of 3:1. Argireline or placebo was applied to their peri-orbital wrinkles twice daily for 4 weeks, and then evaluations were made for the improvements in wrinkles. In the subjective evaluation, Daniell’s classification and Seeman’s standard were applied to make a global assessment of changes in the appearance of peri-orbital lines. In the objective evaluation, silicone replicas of the skin at the application area were made before and after the treatment, which were analyzed by a wrinkle-analysis apparatus. Results In the subjective evaluation, the total anti-wrinkle efficacy in the argireline group was 48.9 %, compared with 0 % in the placebo group. In the objective evaluation, the parameters of roughness were all decreased in the argireline group (p \ 0.01), while no decrease was obvious in the placebo group (p [ 0.05). Conclusions This study showed that argireline had a significant anti-wrinkle effect in Chinese subjects.
📖 Papers frequently viewed together
171 Citations
20 Citations
1 Citations
Glycosaminoglycans (GAGs), especially hyaluronic acid (HA), regulate tissue flexibility, cell motility, and inflammation. Airway smooth muscle cells (ASMCs) of patients with asthma exhibit abnormal HA metabolism, which contributes to inflammation and remodeling. Here, we investigated the effects of glucocorticoids and long-acting β2-agonists (LABAs) on GAG synthesis and HA metabolism by human primary ASMCs. ASMCs were isolated from airway specimens of 10 patients without asthma and 11 patients w...
24 CitationsSource
#1Katharina RöckH-Index: 14
#2Maria GrandochH-Index: 19
Last. Jens W. FischerH-Index: 18
view all 5 authors...
UVB irradiation causes characteristic features of skin aging including remodeling of the dermal extracellular matrix. A key feature during this process is the up-regulation of matrix metalloproteinases and cleavage of collagen. Hyaluronic acid (HA), a major component of the dermal matrix, decreases after chronic UVB exposure. However, the factors that govern the decline of HA synthesis during the course of actinic aging are largely unknown. The aim of the present study was to explore whether col...
63 CitationsSource
#1Jang Hee Oh (SNU: Seoul National University)H-Index: 6
#1Jang Hee Oh (SNU: Seoul National University)H-Index: 2
Last. Jin Ho Chung (SNU: Seoul National University)H-Index: 58
view all 5 authors...
Glycosaminoglycans (GAGs) and proteoglycans (PGs) are involved in various structural functions and physiological regulations in the skin. To investigate the intrinsic ageing-dependent GAG and PG changes and their gender-specific difference, immunohistochemical stains of several GAGs and PGs were performed in sun-protected buttock skin tissues of young and old, male and female (total n = 32) human skin. Stains of alcian blue, hyaluronic acid (HA) and heparan sulphate were reduced in aged skin in ...
69 CitationsSource
#1Dianhua Jiang (Duke University)H-Index: 34
#2Jiurong Liang (Duke University)H-Index: 32
Last. Paul W. Noble (Duke University)H-Index: 86
view all 3 authors...
Accumulation and turnover of extracellular matrix components are the hallmarks of tissue injury. Fragmented hyaluronan stimulates the expression of inflammatory genes by a variety of immune cells at the injury site. Hyaluronan binds to a number of cell surface proteins on various cell types. Hyaluronan fragments signal through both Toll-like receptor (TLR) 4 and TLR2 as well as CD44 to stimulate inflammatory genes in inflammatory cells. Hyaluronan is also present on the cell surface of epithelia...
601 CitationsSource
18 CitationsSource
#1T. G. Tzellos (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 7
#2Ioannis Klagas (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 9
Last. Eleni Papakonstantinou (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 25
view all 9 authors...
Abstract: Extrinsic skin ageing or ‘photoageing’, as opposed to intrinsic skin ageing, is the result of exposure to external factors, mainly ultraviolet irradiation. Glycosaminoglycans (GAG) and particularly hyaluronic acid (HA) are major components of the cutaneous extracellular matrix involved in tissue repair. However, their involvement in extrinsic skin ageing remains elusive. In this study, we investigated the expression of HA and its metabolizing enzymes in photoexposed and photoprotected ...
79 CitationsSource
#1Thrasivoulos Tzellos (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 17
#2Alexander Dionyssopoulos (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 7
Last. Eleni Papakonstantinou (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 25
view all 6 authors...
Background Juvenile hyaline fibromatosis (JHF) is a rare autosomal recessive disease characterized histologically by deposition of hyaline material and clinically by multiple skin lesions. Clarification of the molecular and structural changes involved in JHF skin lesions may unravel targets for pharmacotherapy. Objective We sought to investigate the expression of glycosaminoglycans and their metabolizing enzymes in lesional as compared with lesion-free skin tissue specimens in JHF. Methods Glyco...
12 CitationsSource
#1Ioannis Klagas (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 9
#2Stephanie GouletH-Index: 7
Last. Michael RothH-Index: 60
view all 8 authors...
Glycosaminoglycans (GAG) are essential extracellular matrix molecules which regulate tissue flexibility, a parameter that is reduced in airways of patients with asthma and chronic obstructive pulmonary disease (COPD). We investigated the expression of GAG and their metabolising enzymes in primary human airway smooth muscle cells (ASMC) obtained from healthy donors (controls) and patients with asthma or COPD. Total GAG synthesis was assessed by [ 3 H]-glucosamine incorporation. GAG were isolated,...
74 CitationsSource
#1Eleni Papakonstantinou (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 25
#2George Karakiulakis (A.U.Th.: Aristotle University of Thessaloniki)H-Index: 25
The extracellular matrix (ECM) plays a significant role in the structure and function of the lung. The ECM is a three-dimensional fibre mesh, comprised of various interconnected and intercalated macromolecules, among which are the glycosaminoglycans (GAG). GAG are long, linear and highly charged, heterogeneous polysaccharides that are composed of a variable number of repeating disaccharide units (macromolecular sugars) and most of them, as their name implies, have a sweet taste. In the lung, GAG...
70 CitationsSource
#1David G. Jackson (John Radcliffe Hospital)H-Index: 77
#1David A. Jackson (John Radcliffe Hospital)H-Index: 154
Summary: The lymphatic system is best known for draining interstitial fluid from the tissues and returning it to the blood circulation. However, the lymphatic system also provides the means for immune surveillance in the immune system, acting as conduits that convey soluble antigens and antigen-presenting cells from the tissues to the lymph nodes, where primary lymphocyte responses are generated. One macromolecule that potentially unites these two functions is the large extracellular matrix glyc...
181 CitationsSource
Cited By17
#1Sonali Nagendran (QVH: Queen Victoria Hospital)H-Index: 2
#2Mohammad Javed Ali (LVPEI: L V Prasad Eye Institute)H-Index: 23
Last. Raman Malhotra (QVH: Queen Victoria Hospital)H-Index: 27
view all 4 authors...
The popularity and variety of temporary and permanent periocular aesthetic treatments has increased over the past decade. Patients frequently present to eye clinics with ocular complications and side effects following these treatments, their severity ranging from ocular irritation from dry eyes to visual loss from vascular occlusion. A careful, thorough history is essential, as many patients may not associate aesthetic procedures with ocular complications, and some may be embarrassed to disclose...
#1Alicja Kluczyk (UWr: University of Wrocław)H-Index: 16
#2Julita Ludwiczak (UWr: University of Wrocław)
Last. Remigiusz Bachor (UWr: University of Wrocław)H-Index: 1
view all 7 authors...
Argireline-containing cosmetics attract public interest due to their confirmed reduction of facial wrinkles. Argireline is a peptide that works by inhibiting the release of neurotransmitters in the neuromuscular junction, producing a botox-like effect. Therefore, it is used as a safe needle-free alternative to botox treatment. In this work we investigated the presence of Argireline in cosmetic creams and sera by application of reversed phase liquid chromatography and tandem mass spectrometry (RP...
#1Seng Han Lim (NUS: National University of Singapore)H-Index: 10
#2Himanshu Kathuria (NUS: National University of Singapore)H-Index: 8
Last. Lifeng Kang (USYD: University of Sydney)H-Index: 23
view all 7 authors...
Acetyl-hexapeptide 3 (AHP-3) has good efficacy and safety profile as an anti-wrinkle small peptide. However, its skin permeation is poor due to its hydrophilicity and large molecular weight. 3D printing of personalised microneedles (MN), that contour to the skin surface, offers an attractive alternative for delivery for AHP-3. However, commercially available photocurable resin for 3D printing are not suitable for fabrication of drug loaded delivery systems. In this study, two liquid monomers, na...
3 CitationsSource
#1Seng Han Lim (NUS: National University of Singapore)H-Index: 10
#2Wen Jun Tiew (NUS: National University of Singapore)H-Index: 1
Last. Lifeng Kang (USYD: University of Sydney)H-Index: 23
view all 6 authors...
Acetyl-hexapeptide 3 (AHP-3) is a small peptide with good anti-wrinkle efficacy and safety profile. However, due to its hydrophilicity and high molecular weight, its skin permeation is generally poor. Innovative microneedle (MN) patch such as the curved, flexible or personalised MN patch is a viable avenue to deliver AHP-3. However, the well-researched geometrical relationship of MN on flat MN patch cannot be assumed for these novel MN patches due to a complex mix of axial and shear forces. In t...
10 CitationsSource
#1N. Kawa (Harvard University)
#2H. Dabbous (AUB: American University of Beirut)
Last. N. Soueidan (AUB: American University of Beirut)
view all 4 authors...
When it comes to prevention and non-invasive treatment, periorbital skin is of particular interest, as it is more sensitive than other areas of the body. A variety of topical options are commonly marketed, causing dilution of evidence and difficulty in selecting optimal treatments. For that reason, it is essential for providers to better understand what compounds show clinically proven efficacy but also what products are readily available. Throughout this chapter, we will first explore the basic...
#1J. H. Kim (KNU: Kyungpook National University)H-Index: 1
#2Jin Kyung Seok (KNU: Kyungpook National University)H-Index: 11
Last. Yong Chool Boo (KNU: Kyungpook National University)H-Index: 28
view all 4 authors...
BACKGROUND: Antimelanogenic peptides are potentially useful to treat hyperpigmentation, but many peptides have limited application because of high cost and/or low activity. OBJECTIVES: To identify small and potent peptide inhibitors of cellular melanin synthesis that are useful for cosmetic and medical applications. METHODS: A positional scanning synthetic tetrapeptide combinatorial library was used for screening of potentially active peptides. Antimelanogenic activities of the peptide pools and...
6 CitationsSource
#1Ji Hae AnH-Index: 2
#2Hee Jung LeeH-Index: 8
Last. Donghyun KimH-Index: 1
view all 4 authors...
Background: Hyaluronic acid (HA)-based microneedle patch has recently been studied for wrinkle improvement. Cross-linked HA (CLHA) is widely used in dermal fillers. Acetyl hexapeptide-8 (AHP-8) and epidermal growth factor (EGF) are used for cosmetic ingredients. Objective: This study aimed to verify the efficacy of the CLHA/HA-based patch with microstructure (microneedle patch) containing AHP-8 or EGF. Methods: A total of 52 Korean females were enrolled in a double-blind, randomized, controlled,...
2 CitationsSource
Background: Botulinum toxin (Botox) consists of 7 types of neurotoxins; however, only toxins A and B are used clinically. Botox A is used for several disorders in the field of medicine, particularly in dermatology, for cosmetic purposes. It is produced by the bacterium Clostridium botulinum and can be used as a treatment to reduce the appearance of wrinkles in the upper areas of the face, elevate the eyebrows and treat problems such as hyperhidrosis, lichen simplex, pompholyx (dyshidrotic eczema...
13 CitationsSource
#1Catherine C. Motosko (NYU: New York University)H-Index: 12
#2Anna K. Ault (NYU: New York University)H-Index: 1
Last. Alexes Hazen (NYU: New York University)H-Index: 22
view all 7 authors...
Abstract Background Spin—reporting that distorts the interpretation of results—is not unusual within scientific literature. Objective To appraise strategies of spin among placebo-controlled double-blind clinical trials of topical treatments for photoaged skin. Methods A systematic review of literature was performed to identify placebo-controlled double-blind clinical trials of topical treatments for photoaged skin. A survey of spin strategies was developed and applied to the cohort of identified...
5 CitationsSource
Acetyl hexapeptide 8 (AH8) is a synthetic peptide for anti-wrinkle cosmetics ingredient. It was developed as a mimetic of botox, patternd after N –terminal end of the protein synatosomal-associated protein 25 (SNAP25), a substrate of botulinum toxin. While AH8 has good efficacy and safety profiles, the permeation through the skin is poor. Therefore, we tried to enhance the transdermal delivery of AH8 by using of hyaluonic acid (HA), a linear polysaccharide of N-acetyl glucosamine and glucuronic ...
1 CitationsSource