Applied and Environmental Microbiology
Papers 10,000
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Streptomyces coelicolor A3(2) is used worldwide for genetic studies, and its complete genome sequence was published in 2002. However, as the whole genome of the type strain of S. coelicolor has not been analyzed, the relationship between S. coelicolor A3(2) and the type strain is not yet well known. To clarify differences in their biosynthetic potential, as well as their taxonomic positions, we sequenced whole genomes of S. coelicolor NBRC 12854T and type strains of its closely related species—s...
The development of more sensitive methodologies, capable of quickly detecting and monitoring a microbial population present in a specific biological matrix, as well as performing to allow for the study of all its metabolic changes (e.g., during the formation of biofilm) to occur, is an essential requirement for both well-being and the food industry. Two techniques, in particular, have gained the attention of scientists: The first is “biospeckle”, an optical technique representing an innovative t...
The novel coronavirus disease 2019 (COVID-19) pandemic has brought to light the role of environmental hygiene in controlling disease transmission. Healthcare facilities are hot spots for infectious pathogens where physical distancing and personal protective equipment (PPE) are not always sufficient to prevent disease transmission. Healthcare facilities need to consider adjunct strategies to prevent transmission of infectious pathogens. In combination with current infection control procedures, ma...
#1Kaitlyn E. Kortright (Yale University)H-Index: 8
#2Rachel E. Done (Yale University)
Last. Paul E. Turner (Yale University)H-Index: 34
view all 5 authors...
There is increasing interest in phage therapy as an alternative to antibiotics for treating bacterial infections, especially using phages that select for evolutionary trade-offs between increased phage resistance and decreased fitness traits such as virulence in target bacteria. A vast repertoire of virulence factors allows the opportunistic bacterial pathogen, Shigella flexneri, to invade human gut epithelial cells, replicate intracellularly, and evade host immunity through intercellular spread...
#1Nan Xiang (University of Bremen)H-Index: 1
#2Christiane Hassenrück (University of Bremen)H-Index: 9
Last. Astrid Gärdes (Alfred Wegener Institute for Polar and Marine Research)H-Index: 2
view all 8 authors...
Mutualistic nutrient cycling in the coral-algae symbiosis depends on limited nitrogen (N) availability for algal symbionts. Denitrifying prokaryotes capable of reducing nitrate or nitrite to dinitrogen could thus support coral holobiont functioning by limiting N availability. Octocorals show some of the highest denitrification rates among reef organisms, however little is known about the community structures of associated denitrifiers and their response to environmental fluctuations. Combining 1...
#1Danielle L. Sexton (UBC: University of British Columbia)H-Index: 2
#2Gao Chen (UT: University of Tennessee)H-Index: 9
Last. Elitza I. Tocheva (UBC: University of British Columbia)H-Index: 20
view all 6 authors...
Dehalococcoides mccartyi (Dhc) and Dehalogenimonas spp. (Dhgm) are members of the class Dehalococcoidia, phylum Chloroflexi, characterized by streamlined genomes and a strict requirement for organohalogens as electron acceptors. Here, we used cryo-electron tomography to reveal morphological and ultrastructural features of Dhc strain BAV1 and 'Candidatus Dehalogenimonas etheniformans' strain GP cells at unprecedented resolution. Dhc cells were irregularly shaped discs (890 ± 110 nm long, 630 ± 11...
#1Hisako Hirayama (JAMSTEC: Japan Agency for Marine-Earth Science and Technology)H-Index: 33
#2Yoshihiro Takaki (JAMSTEC: Japan Agency for Marine-Earth Science and Technology)H-Index: 31
Last. Ken Takai (JAMSTEC: Japan Agency for Marine-Earth Science and Technology)H-Index: 78
view all 15 authors...
The Methyloprofundus clade is represented by uncultivated methanotrophic bacterial endosymbionts of deep-sea bathymodiolin mussels, but only a single free-living species has been cultivated to date. This study reveals the existence of free-living Methyloprofundus variants in the Iheya North deep-sea hydrothermal field in the mid-Okinawa Trough. A clade-targeted amplicon analysis of the particulate methane monooxygenase gene (pmoA) detected 647 amplicon sequence variants (ASVs) of the Methyloprof...
#1Jesse McNichol (WHOI: Woods Hole Oceanographic Institution)H-Index: 9
#2Stefan Dyksma (MPG: Max Planck Society)H-Index: 6
Last. Stefan M. Sievert (WHOI: Woods Hole Oceanographic Institution)H-Index: 38
view all 6 authors...
Molecular surveys of low temperature deep-sea hydrothermal vent fluids have shown that Campylobacteria (prev. Epsilonproteobacteria) often dominate the microbial community and that three genera - Arcobacter, Sulfurimonas and Sulfurovum - frequently coexist. In this study, we used replicated radiocarbon incubations of deep-sea hydrothermal fluids to investigate activity of each genus under three experimental conditions. To quantify genus-specific radiocarbon incorporation, we used newly designed ...
#1Gaylen A. Uhlich (USDA: United States Department of Agriculture)H-Index: 14
#2Heather S. Koppenhöfer (USDA: United States Department of Agriculture)
Last. Amy Ream (USDA: United States Department of Agriculture)H-Index: 4
view all 4 authors...
Prophage-encoded Escherichia coli O157:H7 transcription factor (TF), PchE, inhibits biofilm formation and attachment to cultured epithelial cells by reducing curli fimbriae expression and increasing flagella expression. To identify pchE regulators that might be used in intervention strategies to reduce environmental persistence or host infections, we performed a computational search of O157:H7 strain PA20 pchE promoter sequences for binding sites used by known TFs. A common site shared by MarA/S...
#1Shan Zhang (SDU: Shandong University)H-Index: 2
#2Hai-Yan Cao (SDU: Shandong University)H-Index: 5
Last. Chun-Yang Li (Ocean University of China)H-Index: 12
view all 11 authors...
Dimethylsulfoniopropionate (DMSP) is one of the most abundant organic sulfur compounds in the oceans, which is mainly degraded by bacteria through two pathways, a cleavage pathway and a demethylation pathway. Its volatile catabolites dimethyl sulfide (DMS) and methanethiol (MT) in these pathways play important roles in the global sulfur cycle and have potential influences on the global climate. Intense DMS/DMSP cycling occurs in the Arctic. However, little is known about the diversity of cultiva...
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