Molecular Microbiology
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#1Zachary DeMars (KU: University of Kansas)H-Index: 2
#2Christina N. Krute (KU: University of Kansas)H-Index: 8
Last. Jeffrey L. Bose (KU: University of Kansas)H-Index: 19
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In Staphylococcus aureus, the two-component system SaeRS is responsible for regulating various virulence factors essential for the success of this pathogen. SaeRS can be stimulated by neutrophil-derived products but has also recently been shown to be inactivated by the presence of free fatty acids. A mechanism for how fatty acids negatively impacts SaeRS has not been described. We found that unsaturated fatty acids, as well as fatty acids not commonly found in Staphylococcal membranes, prevent t...
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#1Jude Beaudoin (Université de Sherbrooke)H-Index: 18
#2Vincent Normant (Université de Sherbrooke)H-Index: 4
Last. Simon Labbé (Université de Sherbrooke)H-Index: 25
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This study identifies a post-transcriptional mechanism of iron uptake regulation by Puf2 and Puf4 of the Pumilio and FBF (Puf) family of RNA-binding proteins in Schizosaccharomyces pombe. Cells expressing Puf2 and Puf4 stimulate decay of the frp1+ mRNA encoding a key enzyme of the reductive iron uptake pathway. Results consistently showed that frp1+ mRNA is stabilized in puf2Δ puf4Δ mutant cells under iron-replete conditions. As a result, puf2Δ puf4Δ cells exhibit an increased sensitivity to iro...
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#1Nino Iakobachvili (UM: Maastricht University)H-Index: 6
#2Leon-Icaza S (University of Toulouse)
Last. Julien MazieresH-Index: 69
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Respiratory infections remain a major global health concern. Tuberculosis is one of the top 10 causes of death worldwide, while infections with Non-Tuberculous Mycobacteria are rising globally. Recent advances in human tissue modeling offer a unique opportunity to grow different human "organs" in vitro, including the human airway, that faithfully recapitulates lung architecture and function. Here, we have explored the potential of human airway organoids (AOs) as a novel system in which to assess...
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#1Thilina U.B. Herath (University of Otago)
#2Arpita Roy (University of Otago)
Last. Keith Ireton (University of Otago)H-Index: 26
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Shigella flexneri is a gram-negative bacterial pathogen that causes dysentery. Critical for disease is the ability of Shigella to use an actin-based motility (ABM) process to spread between cells of the colonic epithelium. ABM transports bacteria to the periphery of host cells, allowing the formation of plasma membrane protrusions that mediate spread to adjacent cells. Here we demonstrate that efficient protrusion formation and cell-to-cell spread of Shigella involves bacterial stimulation of ho...
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#1Yumeng Chen (ECUST: East China University of Science and Technology)H-Index: 7
#2Wei Wang (ECUST: East China University of Science and Technology)H-Index: 126
Last. Dongzhi Wei (ECUST: East China University of Science and Technology)H-Index: 36
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The filamentous fungus Trichoderma reesei is widely used for industrial cellulase production. In T. reesei, cellulase gene expression is tightly controlled by a regulatory network involving multiple transcription factors. Here, we isolated a novel protein, Rce2, using a pull-down assay and mass spectrometry analysis, from a partial carbon catabolite de-repression mutant, T. reesei Rut-C30, cultured under glucose-repressing conditions. Deletion and overexpression of Rce2 in T. reesei wild-type QM...
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#1Xuejiao Song (UIUC: University of Illinois at Urbana–Champaign)H-Index: 1
#2John E. Cronan (UIUC: University of Illinois at Urbana–Champaign)H-Index: 106
Biotin is an essential metabolic cofactor and de novo biotin biosynthetic pathways are widespread in microorganisms and plants. Biotin synthetic genes are generally found clustered into bio operons to facilitate tight regulation since biotin synthesis is a metabolically expensive process. Dethiobiotin synthetase (DTBS) catalyzes the penultimate step of biotin biosynthesis, the formation of 7,8-diaminononanoate (DAPA). In Escherichia coli, DTBS is encoded by the bio operon gene bioD. Several stud...
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#1Doulin C Shepherd (University of Melbourne)
#2Somavally Dalvi (University of Melbourne)
Last. Debnath Ghosal (University of Melbourne)H-Index: 12
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Electron cryo-microscopy (cryo-EM) has lately emerged as a powerful method in structural biology and cell biology. While cryo-EM single-particle analysis (SPA) is now routinely delivering structures of purified proteins and protein complexes at near-atomic resolution, the use of electron cryo-tomography (cryo-ET), together with subtomogram averaging, is allowing visualization of macromolecular complexes in their native cellular environment, at unprecedented resolution. The unique ability of cryo...
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#1Tina Bedekovic (University of Exeter)
#2Alexandra C. Brand (University of Exeter)H-Index: 14
Advances in microfabrication technology, and its increasing accessibility, allow us to explore fungal biology as never before. By coupling molecular genetics with fluorescence live-cell imaging in custom-designed chambers, we can now probe single yeast cell responses to changing conditions over a lifetime, characterise population heterogeneity and investigate its underlying causes. By growing filamentous fungi in complex physical environments, we can identify cross-species commonalities, reveal ...
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#1Eleanor K. P. Marshall (Imperial College London)
#2Marc Dionne (Imperial College London)H-Index: 34
Animal models have played an essential role in understanding the host-pathogen interactions of pathogenic mycobacteria, including the Mycobacterium tuberculosis and emerging nontuberculous mycobacteria (NTM) species such as Mycobacterium avium and Mycobacterium abscessus. Drosophila melanogaster has become a well-established model for the study of innate immunity and is increasingly being used as a tool to study host-pathogen interactions, in part due to its genetic tractability. Use of D. melan...
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#1J. Kimmel (Bernhard Nocht Institute for Tropical Medicine)
#2Jessica Kehrer (Heidelberg University)H-Index: 8
Last. Tobias Spielmann (Bernhard Nocht Institute for Tropical Medicine)H-Index: 32
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In the last 10 years, proximity-dependent biotinylation (PDB) techniques greatly expanded the ability to study protein environments in the living cell that range from specific protein complexes to entire compartments. This is achieved by using enzymes such as BirA* and APEX that are fused to proteins of interest and biotinylate proteins in their proximity. PDB techniques are now also increasingly used in apicomplexan parasites. In this review, we first give an overview of the main PDB approaches...
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