A Sensitive Competitive ELISA for Determination of Biotin in Transformed Yeast Culture Media
Abstract
Aim To develop a sensitive competitive ELISA for the determination of biotin in transformed yeast culture media.Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae, and then successively incubated with rabbit ami-Mycoplasma hyopneumoniae serum and goat anti-rabbit IgG-biotin to form the solid biotin, which competed with the biotin in the solution (standard or sample) for the limited streptavidin-horse radish peroxidase...
Paper Details
Title
A Sensitive Competitive ELISA for Determination of Biotin in Transformed Yeast Culture Media
Published Date
Jan 1, 2003
Journal
Volume
12
Issue
4
Pages
201 - 206
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