Establishment of a GFP Fluorescence Labeled GLP-1 Receptor Cell Line
Abstract
To establish a green fluorescent protein (GFP)- based cellular model for screening GLP-1 receptor ligands from Traditional Chinese Medicinal herbs. A eukaryotic expression vector pCMV6-GLP-1R-GFP was constructed and transfected into U20S cells. A stable expressing GLP- 1R-GFP U20S cell line was established and the expression of GLP-1 receptor was confirmed by Western Blots with a GLP-1 specific antibody. The activities of expressed GLP-1- GFP...
Paper Details
Title
Establishment of a GFP Fluorescence Labeled GLP-1 Receptor Cell Line
Published Date
Jan 1, 2015
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