Gene

Heart transplantation

Cell biology

Organoid

Induced pluripotent stem cell

Neuroscience

Biology

Biomedical engineering

Transplantation

Cardiology

Biochemistry

Atrium (architecture)

Heart failure

Anatomy

Electrophysiology

Embryonic stem cell

Ventricular assist device

Cardiac function curve

Internal medicine

Medicine

Human heart

Atrial fibrillation

Ventricle

Biomaterials

Currently, due to the increasing demand for 3D culture, various organoids that mimic organs are being actively studied. Despite active reports, information on heart organoids (HOs), which are the first functional organs, is still insufficient. Parameters for reproducing hearts are: chamber formation, organization with cardiac cells, vascularization, and simulation of electrophysiological signals. In particular, since the heart reflects complex factors, it is necessary to develop HOs that can be simulated in depth. In this study, we have created self-organized HOs using human iPSCs, and validated mimicry of cardiac structures such as chamber and epicardium/myocardium and atrium/ventricle-similar areas. Furthermore, mechanical/electrophysiological features were verified through multiple analyzes after inhibition of ion channels. More importantly, the HOs function, due to the cardiovascular characteristics of HOs, was maintained through vascularization after in vivo transplantation. In conclusion, this study has the advantage of being able to easily and closely recapitulate morphological/functional aspects of the heart.

Generation of human iPSCs derived heart organoids structurally and functionally similar to heart