Application of RP-HPLC Technique for Development of Analytical Method for Validation of Albuterol Sulphate from Bulk Drug and Dosage Form

A novel reverse phase liquid chromatographic method was developed and validated for estimation of Albuterol sulphate in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Hypersil BDS C18 (150mm x 4.6mm, 5µm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 75:25 % (v/v). The flow rate was maintained at 1.0ml/min. The detection was carried out at...