Thermoregulation

Gene

Host (biology)

Ectotherm

Animal science

Microbiome

Ecology

Vertebrate

Heat stress

Genetics

Phenotypic plasticity

Biology

Zoology

Nature Ecology & Evolution

Identifying factors that influence how ectothermic animals respond physiologically to changing temperatures is of high importance given current threats of global climate change. Host-associated microbial communities impact animal physiology and have been shown to influence host thermal tolerance in invertebrate systems. However, the role of commensal microbiota in the thermal tolerance of ectothermic vertebrates is unknown. Here we show that experimentally manipulating the tadpole microbiome through environmental water sterilization reduces the host’s acute thermal tolerance to both heat and cold, alters the thermal sensitivity of locomotor performance, and reduces animal survival under prolonged heat stress. We show that these tadpoles have reduced activities of mitochondrial enzymes and altered metabolic rates compared with tadpoles colonized with unmanipulated microbiota, which could underlie differences in thermal phenotypes. These results demonstrate a strong link between the microbiota of an ectothermic vertebrate and the host’s thermal tolerance, performance and fitness. It may therefore be important to consider host-associated microbial communities when predicting species’ responses to climate change. Tadpoles with experimentally manipulated microbiomes have reduced activity of mitochondrial enzymes and altered metabolic rates, resulting in altered thermal sensitivity of locomotion and reduced thermal tolerance and survival.

Experimental manipulation of microbiota reduces host thermal tolerance and fitness under heat stress in a vertebrate ectotherm