Expression and one-step purification of active LPL contemplated by biophysical considerations
Abstract
LPL is essential for intravascular lipid metabolism and is of high medical relevance. Since LPL is notoriously unstable, there is an unmet need for a robust expression system producing high quantities of active and pure recombinant human LPL (hLPL). We showed previously that bovine LPL purified from milk is unstable at body temperature (Tm is 34.8°C), but in the presence of the endothelial transporter glycosylphosphatidylinositol-anchored high...
Paper Details
Title
Expression and one-step purification of active LPL contemplated by biophysical considerations
Published Date
Jan 1, 2021
Journal
Volume
62
Pages
100149 - 100149
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