Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
Abstract
Functional genomics studies of the immune system require genetic manipulations that involve both deletion of target genes and addition of elements to proteins of interest. Identification of gene functions in cell line models is important for gene discovery and exploration of cell-intrinsic mechanisms. However, genetic manipulations of immune cells such as T cells and macrophage cell lines using CRISPR/Cas9-mediated knock-in are difficult because...
Paper Details
Title
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
Published Date
Nov 13, 2021
Issue
177
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