Gene

RNA-Seq

Deep sequencing

Genome

Gene expression

Transcriptome

Exome sequencing

Single cell sequencing

Genomics

Mutation

DNA sequencing

Genetics

RNA splicing

Computational biology

Biology

International Journal of Oral Science

RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNAseq), popular single cell RNA sequencing (scRNAseq) to newly emerged spatial RNA sequencing (spRNAseq). Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing. This article highlights these technologies, characteristic features and suitable applications in precision oncology.

From bulk, single-cell to spatial RNA sequencing