In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

Volume: 57, Issue: 96, Pages: 12980 - 12983
Published: Jan 1, 2021
Abstract
The membrane transporter BtuB is site-directedly spin labelled on the surface of living Escherichia coli via Diels-Alder click chemistry of the genetically encoded amino acid SCO-L-lysine. The previously introduced photoactivatable nitroxide PaNDA prevents off-target labelling, is used for distance measurements, and the temporally shifted activation of the nitroxide allows for advanced experimental setups. This study describes significant...
Paper Details
Title
In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code
Published Date
Jan 1, 2021
Volume
57
Issue
96
Pages
12980 - 12983
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