Optimization of solvent extraction and HPLC-DAD method parameters for determination of phenolic compounds in various Brazilian propolis

Propolis is an organotherapeutic product collected by honey bees that contains a large number of phytoconstituents, possessing excellent pharmacological properties. In this study, phenolic composition and antioxidant capacity were evaluated in different types of Brown, Green and mixture of Red, Green I and Brown II (RGB) Brazilian propolis. Ultrasonic-assisted extraction was performed by direct solvent extraction (DSE) and successive solvent...