Bottom-up fabrication of a proteasome–nanopore that unravels and processes single proteins
Abstract
The precise assembly and engineering of molecular machines capable of handling biomolecules play crucial roles in most single-molecule methods. In this work we use components from all three domains of life to fabricate an integrated multiprotein complex that controls the unfolding and threading of individual proteins across a nanopore. This 900 kDa multicomponent device was made in two steps. First, we designed a stable and low-noise β-barrel...
Paper Details
Title
Bottom-up fabrication of a proteasome–nanopore that unravels and processes single proteins
Published Date
Nov 18, 2021
Journal
Volume
13
Issue
12
Pages
1192 - 1199
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Notes
History