LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian.

Published on Sep 1, 2021in Asian Pacific Journal of Cancer Prevention
· DOI :10.31557/APJCP.2021.22.9.2819
Heba Morsy7
Estimated H-index: 7
(Alexandria University),
Ahmed Gaballah7
Estimated H-index: 7
(Alexandria University)
+ 6 AuthorsWaleed Arafat18
Estimated H-index: 18
(Alexandria University)
Objective null The study aimed to delineate the gene expression profile of LGR5, HES1 and ATOH1 in young Egyptian rectal cancer (RC) patients and investigate the correlation between expression profiles and clinical outcome. null Methods null The study was conducted on 30 young Egyptian RC patients. Expression study of LGR5, HES1 and ATOH1 were performed by quantitative PCR (QPCR) based on comparative Cq method after normalization to adjacent non tumor tissues and ACTB as a reference gene. Patients were followed up for assessment of response to neoadjuvant chemoradiotherapy (CRT) based on revised RECIST1.1. null Result null The study detected overexpression of LGR5 and HES1 and down-regulation of ATOH1 in human RC tissues compared to non- tumor tissues. High expression of LGR5 was correlated with more depth of tumor invasion, lymph node (LN) metastasis, advanced cTNM stage and mesorectal fascia (MRF) involvement. More prominently, high LGR5 expression level was associated with poor response to CRT. LGR5 was suggested as unfavorable prognostic biomarker for RC. Conversely, HES1 and ATOH1 expression did not show significant association with most of the studied clinical criteria nor response to CRT. Still, HES1 and ATOH1 were significantly and inversely associated with presence of mucinous component. null Conclusion null High LGR5 expression is indicative of poor prognosis among young Egyptian RC patients and is proposed as a predictive marker of resistance to neoadjuvant CRT. However, HES1 and ATOH1 expressions were not prognostic nor predictive of response to CRT. Overall, LGR5, HES1 and ATOH1 gene expression patterns among young onset RC patients, are in line with patterns encountered in older age groups.
#1Rama Krishna Nimmakayala (UNMC: University of Nebraska Medical Center)H-Index: 9
#2Surinder K. BatraH-Index: 99
Last. Moorthy P. PonnusamyH-Index: 34
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Abstract Cancer biology research over recent decades has given ample evidence for the existence of self-renewing and drug-resistant populations within heterogeneous tumors, widely recognized as cancer stem cells (CSCs). However, a lack of clear understanding about the origin, existence, maintenance, and metastatic roles of CSCs limit efforts towards the development of CSC-targeted therapy. In this review, we describe novel avenues of current CSC biology. In addition to cell fusion and horizontal...
#1Bo Gun Jang (Jeju National University)H-Index: 4
#2Hye Sung Kim (Jeju National University)H-Index: 7
Last. Gyeong Hoon Kang (SNU: Seoul National University)H-Index: 66
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We investigated the expression profile of leucine-rich, repeat-containing, G-protein–coupled receptor 5 (LGR5) during colorectal cancer (CRC) progression and determined the prognostic impact of LGR5 in a large cohort of CRC samples. LGR5 expression was higher in CRCs than in normal mucosa, and was not associated with other cancer stem cell markers. LGR5 positivity was observed in 68% of 788 CRCs and was positively correlated with older age, moderately to well-differentiated cells, and nuclear β-...
#1Weidong Wu (SJTU: Shanghai Jiao Tong University)H-Index: 10
#2Jun Cao (SJTU: Shanghai Jiao Tong University)H-Index: 17
Last. Hong-hua Ding (SJTU: Shanghai Jiao Tong University)H-Index: 3
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// Weidong Wu 1, * , Jun Cao 1, * , Zhengyi Ji 1 , Jingjue Wang 2 , Tao Jiang 1 , Honghua Ding 2 1 Department of Gastrointestinal Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China 2 Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China * These authors contributed equally to this work Correspondence to: Honghua Ding, email: dinghonghua_16@126.com , dinghonghua@163.com T...
#1Mahsa Ahadi (Kolling Institute of Medical Research)H-Index: 8
#2Juliana Andrici (Kolling Institute of Medical Research)H-Index: 21
Last. Anthony J. Gill (Kolling Institute of Medical Research)H-Index: 76
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Alterations in the Notch signaling pathway play a role in colorectal cancer (CRC). Hes1, a Notch-induced transcription factor, has recently been reported to show decreased expression by immunohistochemistry in sessile serrated adenomas. Variable staining patterns have been reported in tubular adenomas, and existing data on Hes1 expression in CRC are limited and inconsistent. We therefore sought to investigate the expression of Hes1 by immunohistochemistry in a large and well-characterized cohort...
#1Yangyan Jiang (ZJU: Zhejiang University)H-Index: 2
#2Wenlu Li (ZJU: Zhejiang University)H-Index: 14
Last. Zhigang Chen (ZJU: Zhejiang University)H-Index: 20
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Background Lgr5 has recently been identified as a reliable biomarker of cancer stem cells (CSCs) in colorectal cancer (CRC); however, its prognostic value is still controversial.
#1Andrius K. Planutis (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 1
#2Randall F. HolcombeH-Index: 23
Last. Kiastoutis S. PlanoutisH-Index: 1
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Leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5) is a colorectal cancer (CRC) stem cell marker. The role of Lgr5-expressing stem cells in resistance to chemotherapy is controversial. The notion that Lgr5-expressing cells are more chemotherapy resistant is supported by some data; other data do not support this notion. We hypothesized that Lgr5-expressing cells would be more chemotherapy sensitive, as Lgr5 is usually a marker of dividing cells. We tested this hypothesis by exploi...
#1Keita Fukushima (Tokyo Medical and Dental University)H-Index: 5
#2Kiichiro Tsuchiya (Tokyo Medical and Dental University)H-Index: 31
Last. Mamoru Watanabe (Tokyo Medical and Dental University)H-Index: 87
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Patients with inflammatory bowel disease (IBD) have an increased risk of developing colitis-associated colorectal cancer (CAC). CAC cells often develop chemoresistance, resulting in a poorer prognosis than that of sporadic colorectal cancer (CRC). The mechanism by which CAC enhances malignant potential remains unknown. We have previously reported that the proteasomal degradation of the transcription factor Atonal homolog 1 (Atoh1) protein results in the non-mucinous form of CRC. It also remains ...
#1Fei Gao (JNU: Jinan University)H-Index: 16
#2Yuqin Zhang (Southern Medical University)H-Index: 8
Last. Dong Xiao (Southern Medical University)H-Index: 15
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A small subpopulation of cancer cells with stem cell-like features might be responsible for tumour generation, progression, and chemoresistance. Hes1 influences the maintenance of certain stem cells and progenitor cells and the digestive systems. We found upregulated Hes1 in poorly differentiated cancer samples compared with well-differentiated tumour samples, and most of the adenocarcinomas exhibited significantly higher levels of Hes1 mRNA compared with that observed in matched normal colon sa...
#1Ye Han (Soochow University (Suzhou))H-Index: 13
#2Xiaofeng Xue (Soochow University (Suzhou))H-Index: 13
Last. Hong Zhao (Soochow University (Suzhou))H-Index: 11
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Background Leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) has been identified as a putative intestinal stem cell marker. However, the clinical prognosis of Lgr5 is still controversial in colorectal cancer (CRC). To systematically summarize the clinical prognostic function of Lgr5 in colorectal cancer, we performed this meta-analysis. Methods Published articles which assessed the clinical or prognostic role of Lgr5 was searched in Pubmed, Embase and Springer and collected unti...
#1Chee Wee Ong ('QUB': Queen's University Belfast)H-Index: 23
#2Pei Yi Chong (NUS: National University of Singapore)H-Index: 5
Last. Manuel Salto-TellezH-Index: 67
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Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in the Western world. It is becoming increasingly clear that CRC is a diverse disease, as exemplified by the identification of subgroups of CRC tumours that are driven by distinct biology. Recently, a number of studies have begun to define panels of diagnostically relevant markers to align patients into individual subgroups in an attempt to give information on prognosis and treatment response. We examined the immunohist...
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