In vivo hyperCEST imaging: Experimental considerations for a reliable contrast

Purpose HyperCEST contrast relies on the reduction of the solvent signal after selective saturation of the solute magnetization. The scope of this work is to outline the experimental conditions needed to obtain a reliable hyperCEST contrast in vivo, where the “solvent” signal (ie, the dissolved‐phase signal) may change over time due to the increase in xenon (Xe) accumulation into tissue. Methods Hyperpolarized 129 Xe was delivered to mice at a...