Application of a statistical design to evaluate bioethanol production from Chlorella S4 biomass after acid - Thermal pretreatment

This research evaluates the potential of a native marine isolate, Chlorella S4, for bioethanol production using the Thermo-Tolerant (TT) strain of Saccharomyces cerevisiae. Initially, an acid-thermal pretreatment with different concentrations of microalgal biomass (2, 4, and 6% (w/v)) and H2SO4 (1.5, 3, and 4.5% (v/v)) was investigated to improve the release of glucose (an important substrate of fermentation). The glucose concentration was...