Biomedical engineering

Diffuse optical imaging

Chemistry

Biochemistry

Physics

Optics

Acoustics

Optical coherence tomography

Computer science

Dynamics (music)

Microscopy

Tomography

Spheroid

In vitro

Medicine

Biomedical Optics Express

We present a completely label-free three-dimensional (3D) optical coherence tomography (OCT)-based tissue dynamics imaging method for visualization and quantification of the metabolic and necrotic activities of tumor spheroid. Our method is based on a custom 3D scanning protocol that is designed to capture volumetric tissue dynamics tomography images only in a few tens of seconds. The method was applied to the evaluation of a tumor spheroid. The time-course viability alteration and anti-cancer drug response of the spheroid were visualized qualitatively and analyzed quantitatively. The similarity between the OCT-based dynamics images and fluorescence microscope images was also demonstrated.

Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation