Amorphous calcium phosphate nanoparticles using adenosine triphosphate as an organic phosphorus source for promoting tendon-bone healing.

Published on Sep 8, 2021in Journal of Nanobiotechnology10.435
· DOI :10.1186/S12951-021-01007-Y
Haoran Liao (SJTU: Shanghai Jiao Tong University), Han-Ping Yu5
Estimated H-index: 5
(CAS: Chinese Academy of Sciences)
+ 5 AuthorsYaohua He20
Estimated H-index: 20
(SJTU: Shanghai Jiao Tong University)
Sources
Abstract
Background null Rotator cuff tear (RCT) is a common problem of the musculoskeletal system. With the advantage of promoting bone formation, calcium phosphate materials have been widely used to augment tendon-bone healing. However, only enhancing bone regeneration may be not enough for improving tendon-bone healing. Angiogenesis is another fundamental factor required for tendon-bone healing. Therefore, it's necessary to develop a convenient and reliable method to promote osteogenesis and angiogenesis simultaneously, thereby effectively promoting tendon-bone healing. null Methods null The amorphous calcium phosphate (ACP) nanoparticles with dual biological activities of osteogenesis and angiogenesis were prepared by a simple low-temperature aqueous solution method using adenosine triphosphate (ATP) as an organic phosphorus source. The activities of osteogenesis and angiogenesis and the effect on the tendon-bone healing of ACP nanoparticles were tested in vitro and in a rat model of acute RCT. null Results null The ACP nanoparticles with a diameter of tens of nanometers were rich in bioactive adenosine. In vitro, we confirmed that ACP nanoparticles could enhance osteogenesis and angiogenesis. In vivo, radiological and histological evaluations demonstrated that ACP nanoparticles could enhance bone and blood vessels formation at the tendon-bone junction. Biomechanical testing showed that ACP nanoparticles improved the biomechanical strength of the tendon-bone junction and ultimately promoted tendon-bone healing of rotator cuff. null Conclusions null We successfully confirmed that ACP nanoparticles could promote tendon-bone healing. ACP nanoparticles are a promising biological nanomaterial in augmenting tendon-bone healing.
References36
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#1Yao Huang (Nanjing University of Chinese Medicine)H-Index: 3
#2Bing He (Nanjing University of Chinese Medicine)H-Index: 3
Last. Luning Sun (Nanjing University of Chinese Medicine)H-Index: 3
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Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator cuff reconstruction, but the process of tendon-bone healing is complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method to promote tendon-bone healing. MSCs have strong paracrine, anti-inflammatory, immunoregulatory, and angiogenic potential. Recent studies have shown that MSCs achieve many regulatory functions through exosomes. The purpo...
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#1Dong Min Kim (UOU: University of Ulsan)H-Index: 4
#2In Kyoung Shim (UOU: University of Ulsan)H-Index: 1
Last. Kyoung Hwan Koh (UOU: University of Ulsan)H-Index: 24
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Background:Tearing and degeneration of the rotator cuff at the tendon-to-bone junction are common in adults aged ≥50 years. Few studies have reported on the relationship between estrogen and the ro...
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BACKGROUND Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. HYPOTHESIS Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. STUDY DESIGN Controlled laboratory study. ...
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#1Yao Huang (Nanjing University of Chinese Medicine)H-Index: 3
#2Min PanH-Index: 1
Last. Luning Sun (Nanjing University of Chinese Medicine)H-Index: 3
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Local angiogenesis following rotator cuff reconstruction is crucial for tendon-bone healing. The current research on the mechanism underlying angiogenesis that promotes tendon-bone healing is scarce. This study investigates the mechanism underlying vascular endothelial growth factor (VEGF)-Hippo signaling pathway's involvement in tendon-bone healing following rotator cuff reconstruction. Verteporfin, the inhibitor of the Yes-associated protein (YAP), was used to mechanically test and analyze two...
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#1Rui Sun (Uppsala University)H-Index: 10
#2Michelle Åhlén (Uppsala University)H-Index: 4
Last. Ocean Cheung (Uppsala University)H-Index: 15
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Amorphous calcium phosphate (ACP) has shown significant effects on the biomineralization and promising applications in bio-medicine. However, the limited stability and porosity of ACP material restrict its practical applications. A storage stable highly porous ACP with Brunauer–Emmett–Teller surface area of over 400 m2/g was synthesized by introducing phosphoric acid to a methanol suspension containing amorphous calcium carbonate nanoparticles. Electron microscopy revealed that the porous ACP wa...
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#1Shasha Yao (ZJU: Zhejiang University)H-Index: 5
#2Xianfeng Lin (SRRSH: Sir Run Run Shaw Hospital)H-Index: 1
Last. Ruikang Tang (ZJU: Zhejiang University)H-Index: 46
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Osteoporosis is an incurable chronic disease characterized by a lack of mineral mass in the bones. Here, the full recovery of osteoporotic bone is achieved by using a calcium phosphate polymer-induced liquid-precursor (CaP-PILP). This free-flowing CaP-PILP material displays excellent bone inductivity and is able to readily penetrate into collagen fibrils and form intrafibrillar hydroxyapatite crystals oriented along the c-axis. This ability is attributed to the microstructure of the material, wh...
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#2Stephen F. Brockmeier (University of Virginia Health System)H-Index: 25
Last. Brian C. Werner (University of Virginia Health System)H-Index: 39
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Purpose To examine any association between osteoporosis and the failure of arthroscopic rotator cuff repair (ARCR) leading to revision surgery and to investigate whether the use of bisphosphonates had any influence on the observed associations. Methods Patients who underwent ARCR with a diagnosis of osteoporosis were identified from the PearlDiver database and stratified according to whether there was a filled prescription for a bisphosphonate in the perioperative period. Patients with osteoporo...
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#1Xiebo You (SJTU: Shanghai Jiao Tong University)H-Index: 1
#2Yueqin Shen (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Yaohua He (SJTU: Shanghai Jiao Tong University)H-Index: 20
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: The formation of fibrocartilage at the healing site following a rotator cuff tear repair is a major problem in the field of tendon‑bone healing. The present study aimed to enhance the healing of the tendon‑bone interface following rotator‑cuff tear repair by the interposition of hydroxyapatite (HA) encapsulated with transforming growth factor β1 (TGFβ1). Using an acute rotator cuff repair model, rats were divided into three groups: i) Repair only (control); ii) HA group; and iii) HA‑TGFβ1 grou...
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#1Song Zhao (SJTU: Shanghai Jiao Tong University)H-Index: 10
#2Wei Su (SJTU: Shanghai Jiao Tong University)H-Index: 1
Last. Xin Zhao (PolyU: Hong Kong Polytechnic University)H-Index: 82
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Abstract Tearing of the rotator cuff commonly occurs as among one of the most frequently experienced tendon disorders. While treatment typically involves surgical repair, failure rates to achieve or sustain healing range from 20 to 90%. The insufficient capacity to recover damaged tendon to heal to the bone, especially at the enthesis, is primarily responsible for the failure rates reported. Various types of biomaterials with special structures have been developed to improve tendon-bone healing ...
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#1Shivam A. Shah (WashU: Washington University in St. Louis)H-Index: 6
#2Ioannis KormpakisH-Index: 6
Last. Stavros ThomopoulosH-Index: 62
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Abstract Rotator cuff tears are a common source of pain and disability, and poor healing after repair leads to high retear rates. Bone loss in the humeral head before and after repair has been associated with poor healing. The purpose of the current study was to mitigate bone loss near the repaired cuff and improve healing outcomes. Sclerostin antibody (Scl-Ab) treatment, previously shown to increase bone formation and strength in the setting of osteoporosis, was used in the current study to add...
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