Improved extraction and purification of the hydrophobin HFBI

Hydrophobins (HFBs) are a group of highly functional, low molecular weight proteins with the ability to self‐assemble at hydrophobic‐hydrophilic interfaces. The surface active, cysteine‐rich proteins are found in filamentous fungi such as Trichoderma reesei . In the present study multiple extraction solvents and conditions were screened for the mycelium bound hydrophobin HFBI and the effects on the total amount of extracted proteins, HFBI...