Assessment of Biphasic Extraction Methods of Mouse Fecal Metabolites for Liquid Chromatography–Mass Spectrometry-Based Metabolomic Studies

With the increasing knowledge about the important roles of gut microbiota on the biological system, a systematic strategy to profile the fecal metabolome is urgently needed. Thus, an unbiased, efficient, and reproducible fecal metabolite extraction protocol needs to be established; however, the effect of biphasic extraction methods for the fecal samples remains unclear. In this study, five different methods were assessed in the extraction of...