Biocatalytic synthesis of 2‐O‐α‐D‐glucopyranosyl‐L‐ascorbic acid using an extracellular expressed α‐glucosidase from Oryza sativa

Background 2‐ O ‐ α ‐D‐Glucopyranosyl‐L‐ascorbic acid (AA‐2G) is an important derivative of L‐ascorbic acid (L‐AA), which has the distinct advantages of non‐reducibility, antioxidation, and reproducible decomposition into L‐AA and glucose. Enzymatic synthesis is a preferred method for AA‐2G production over alternative chemical synthesis owing to the regioselective glycosylation reaction. α ‐Glucosidase, an enzyme classed into O ‐glycoside...