Targeted and untargeted characterization of underivatized policosanols in hemp inflorescence by liquid chromatography-high resolution mass spectrometry

Published on Dec 1, 2021in Talanta5.339
· DOI :10.1016/J.TALANTA.2021.122778
Carmela Maria Montone12
Estimated H-index: 12
(Sapienza University of Rome),
Sara Elsa Aita2
Estimated H-index: 2
(Sapienza University of Rome)
+ 7 AuthorsAnna Laura Capriotti38
Estimated H-index: 38
(Sapienza University of Rome)
Source
Abstract
Abstract null null The paper describes the development of a targeted quantitative method for the analysis of policosanols in hemp inflorescence. Policosanols are long chain aliphatic alcohols, with carbon chains typically in the range 20–36, with interesting biological activities. These compounds are typically separated by gas chromatography and only a few methods employ liquid chromatography for policosanols. In both cases, methods always include the derivatization of policosanols. In this study, policosanols were separated by ultra-high performance liquid chromatography without any derivatization and detected using high resolution mass spectrometry by formation of lithiated adducts. The procedure was optimized and a quantitative method was validated for the most abundant policosanols (with C24, C26, C27, C28, and C30 chain lengths) in industrial hemp inflorescence extracts. The method was used for the quantitative analysis of policosanols in five hemp types. Hemp wax was found rich in these compounds, especially C26 and C28 policosanols, which may prove useful for revalorization of wax by-products. Finally, the acquired data were also used to expand the search to the untargeted qualitative analysis of policosanols using Compound Discoverer. The untargeted method allowed the annotation of underivatized policosanols up to C33.
References32
Newest
#1Arrigo F.G. Cicero (UNIBO: University of Bologna)H-Index: 67
#2Sergio D'Addato (UNIBO: University of Bologna)H-Index: 22
Last. Claudio Borghi (UNIBO: University of Bologna)H-Index: 42
view all 3 authors...
Phytosterols and red yeast rice are largely studied cholesterol-lowering nutraceuticals, respectively inhibiting the bowel absorption and liver synthesis of cholesterol. Our aim was to test the effect of combined nutraceutical-containing phytosterols and red yeast rice vs. a placebo on the lipid profile. We performed a parallel arms, double-blind, placebo-controlled clinical trial, randomizing 88 moderately hypercholesterolemic subjects to treatment with a combined nutraceutical containing phyto...
3 CitationsSource
Liquid chromatography coupled to quadrupole-based tandem mass spectrometry (QqQ) is termed the "gold standard" for bioanalytical applications because of its unpreceded selectivity, sensitivity, and the ruggedness of the technology. More recently, however, high-resolution mass spectrometry (HRMS) has become increasingly popular for bioanalytical applications. Nonetheless, this technique is still viewed, either as a screening technology or as a research tool. Although HRMS is actively discussed du...
15 CitationsSource
#1Tae Jin Kim (Incheon National University)H-Index: 5
#2Jeong Gon Park (Incheon National University)H-Index: 2
Last. Jae Kwang Kim (Incheon National University)H-Index: 10
view all 8 authors...
Perilla and sesame are traditional sources of edible oils in Asian and African countries. In addition, perilla and sesame seeds are rich sources of health-promoting compounds, such as fatty acids, tocopherols, phytosterols and policosanols. Thus, developing a method to determine the geographic origin of these seeds is important for ensuring authenticity, safety and traceability and to prevent cheating. We aimed to develop a discriminatory predictive model for determining the geographic origin of...
3 CitationsSource
#1Ji-Eun Ra (Konkuk University)H-Index: 1
#2So-Yeun Woo (RDA: Rural Development Administration)H-Index: 1
Last. Woo Duck Seo (RDA: Rural Development Administration)H-Index: 15
view all 10 authors...
Abstract Policosanols is a health promoting aliphatic alcohol known as lipid-lowing agent. To enable maximising the functional properties of wheat, this research investigates the policosanol profiles and adenosine 5′-monophosphate-activated protein kinase (AMPK) activation potential of Korean wheat seedlings according to cultivars and growth times. GC-MS revealed six policosanols that differed markedly in content between 17 cultivars, especially, octacosanol (8) showed the most predominant compo...
3 CitationsSource
#1Ivan AloisiH-Index: 4
#2Mariosimone ZoccaliH-Index: 14
Last. Luigi MondelloH-Index: 69
view all 5 authors...
The present research is focused on the development of a cryogenically modulated (CM) comprehensive two-dimensional gas chromatography-high resolution time-of-flight mass spectrometry (GC × GC-HR ToFMS) method for the in-depth profiling of the unsaponifiable fraction of vegetable oils. Specifically, twelve vegetable oils (9 extra-virgin olive, soybean, peanut, and hazelnut oils) were subjected to analysis, with particular attention devoted to the higher molecular weight constituents (sterols, vit...
7 CitationsSource
#1Wanshu Qi (CAS: Chinese Academy of Sciences)H-Index: 4
#2Yunjun Wang (Fudan University)H-Index: 6
Last. Yinlong Guo (CAS: Chinese Academy of Sciences)H-Index: 26
view all 8 authors...
In this work, we developed a rapid and high-sensitivity method for simultaneous analyses of fatty alcohols, fatty aldehydes, and sterols by combining the optimized derivatization reaction with electrospray ionization-ion mobility-mass spectrometry (ESI-IM-MS). Pyridine and thionyl chloride were used as derivatization reagents as they were easily removed after the derivatization reaction and could generate permanently charged tags on different functional groups including hydroxyls and aldehydes. ...
9 CitationsSource
#1Cinzia CittiH-Index: 11
#2Fabiana Russo (University of Modena and Reggio Emilia)H-Index: 3
Last. Giuseppe Cannazza (University of Modena and Reggio Emilia)H-Index: 24
view all 11 authors...
The chemical analysis of cannabis potency involves the qualitative and quantitative determination of the main phytocannabinoids: Delta(9)-tetrahydrocannabinol (Delta(9)-THC), cannabidiol (CBD), cannabigerol (CBG), cannabichromene (CBC), etc. Although it might appear as a trivial analysis, it is rather a tricky task. Phytocannabinoids are present mostly as carboxylated species at the aromatic ring of the resorcinyl moiety. Their decarboxylation caused by heat leads to a greater analytical variabi...
18 CitationsSource
#1Anneli Kruve (UT: University of Tartu)H-Index: 18
About ten years ago most of the liquid chromatography (LC) electrospray (ESI) mass spectrometry (MS) analysis of environmental, metabolomics, pharmaceutical samples were predominantly carried out a...
22 CitationsSource
#1Cinzia Citti (University of Modena and Reggio Emilia)H-Index: 11
#2Pasquale Linciano (University of Modena and Reggio Emilia)H-Index: 15
Last. Giuseppe Cannazza (University of Modena and Reggio Emilia)H-Index: 24
view all 12 authors...
(-)-Trans-Δ9-tetrahydrocannabinol (Δ9-THC) is the main compound responsible for the intoxicant activity of Cannabis sativa L. The length of the side alkyl chain influences the biological activity of this cannabinoid. In particular, synthetic analogues of Δ9-THC with a longer side chain have shown cannabimimetic properties far higher than Δ9-THC itself. In the attempt to define the phytocannabinoids profile that characterizes a medicinal cannabis variety, a new phytocannabinoid with the same stru...
62 CitationsSource
#1Cinzia Citti (University of Modena and Reggio Emilia)H-Index: 11
#2Pasquale Linciano (University of Modena and Reggio Emilia)H-Index: 15
Last. Giuseppe Cannazza (University of Modena and Reggio Emilia)H-Index: 24
view all 7 authors...
Abstract Cannabidiol (CBD), one of the two major active principles present in Cannabis sativa, is gaining great interest among the scientific community for its pharmaceutical, nutraceutical and cosmetic applications. CBD can be prepared either by chemical synthesis or extraction from Cannabis sativa (hemp). The latter is more convenient from several points of view, including environmental and economic, but mainly for the absence of harmful organic solvents generally employed in the chemical synt...
32 CitationsSource
Cited By0
Newest