Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia.

Published on Aug 13, 2021in BMC Genomics3.594
· DOI :10.1186/S12864-021-07923-5
Shengxing Li1
Estimated H-index: 1
,
Zhuogong Shi1
Estimated H-index: 1
+ 3 AuthorsZhiheng Zhao1
Estimated H-index: 1
Source
Abstract
BACKGROUND Chestnut seeds are important kinds of edible nuts rich in starch and protein. The characteristics and nutrient contents of chestnut have been found to show obvious metaxenia effects in previous studies. To improve the understanding of the effect of metaxenia on chestnut starch and sucrose metabolism, this study used three varieties of chestnut, 'Yongfeng 1', 'YongRen Zao' and 'Yimen 1', as male parents to pollinate the female parent, 'Yongfeng 1', and investigated the mechanisms of starch and sucrose metabolism in three starch accumulation stages (70 (S1), 82 (S2), and 94 (S3) days after pollination, DAP) in chestnut seed kernels. RESULT Most carbohydrate metabolism genes were highly expressed in YFF (self-pollinated 'Yongfeng 1') in stage S2 and in YFR ('Yongfeng 1' × 'Yongren Zao') and YFM ('Yongfeng 1' × 'Yimen 1') in stage S3. In stage S3, hub genes encoding HSF_DNA-binding, ACT, Pkinase, and LIM proteins and four transcription factors were highly expressed, with YFF showing the highest expression, followed by YFR and YFM. In addition, transcriptome analysis of the kernels at 70, 82 and 94 DAP showed that the starch granule-bound starch synthase (EC 2.4.1.242) and ADP-glucose pyrophosphorylase (EC 2.7 .7.27) genes were actively expressed at 94 DAF. Chestnut seeds regulate the accumulation of soluble sugars, reducing sugars and starch by controlling glycosyl transferase and hydrolysis activity during development. CONCLUSION These results and resources have important guiding significance for further research on starch and sucrose metabolism and other types of metabolism related to chestnut metaxenia.
References33
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#1Shengxing LiH-Index: 1
#2Haiying LiangH-Index: 1
Last. Zhiheng ZhaoH-Index: 1
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Chestnuts are popular edible nuts that are rich in starch. In order to enhance the transcriptomic resources and further understand starch and sucrose metabolism in maturing chestnuts, a comparative transcriptomic study of Chinese chestnut kernels was conducted at three ripening stages (70, 82, and 94 DAF). At 82 and 94 days after flowering (DAF), starch continued to accumulate, and the amylopectin/amylose ratio increased. Transcriptomic profiling of kernels at 70 (stage I), 82 (stage II), and 94...
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#2Neriman BeyhanH-Index: 8
This study has been conducted between 2015 and 2016 at Hazelnut Research Institute in Giresun. The objective of this study was to determine the effects of the pollinizer cultivars on the nut and kernel characteristics. Tombul, Palaz, Cakildak, Fosa and Allahverdi were used as main cultivars while Tombul, Palaz, Cakildak, Fosa, Allahverdi, Sivri, Kalinkara and Yassi Badem were used as pollinizer cultivars. Self-pollination was considered as a control and open-pollination was taken as an applicati...
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#3Qing Zhang (PKU: Peking University)
BACKGROUND: The Chinese chestnut (Castanea mollissima) is widely cultivated in China for nut production. This plant also plays an important ecological role in afforestation and ecosystem services. To facilitate and expand the use of C. mollissima for breeding and its genetic improvement, we report here the whole-genome sequence of C. mollissima. FINDINGS: We produced a high-quality assembly of the C. mollissima genome using Pacific Biosciences single-molecule sequencing. The final draft genome i...
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#7Qing Zhang (PKU: Peking University)
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#1Feng Zou (BFU: Beijing Forestry University)H-Index: 1
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#1Lin Zhang (Central South University Forestry and Technology)H-Index: 17
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The Chinese chestnut (Castanea mollissima) seed provides a rich source of carbohydrates as food and feed. However, little is known about starch biosynthesis in the seeds. The objectives of this study were to determine seed composition profiles and identify genes involved in starch and sucrose metabolism. Metabolite analysis showed that starch was the major component and rapidly accumulated during seed endosperm development. Amylopectin was approximately 3-fold of amylose content in chestnut star...
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Xenia, the transmission of traits from the pollinizer to the female's tissues, is a phenomenon hitherto unknown in tomatoes. Here, we describe xenia effects on the seeds and fruits of Solanum lycopersicum, the tomato, elicited by S. galapagense. The wild tomatoes, such as S. galapagense, have highly pilose fruit surface and minute seeds, unlike the domesticated species. Crossings between S. galapagense (pollinizer) and two large-seeded, glabrous cultivars of S. lycopersicum (females) tested the ...
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