Production, Purification and Characterization of ‘Iturin A-2’ a Lipopeptide with Antitumor Activity from Chinese Sauerkraut Bacterium Bacillus velezensis T701

This study was done to purify and characterize antitumor lipopeptide from Bacillus strains. Bacillus velezensis strain T701, which was isolated from Chinese sauerkraut, was screened out for its antitumor activity of lipopeptide in high level against breast cancer cells BT474. The lipopeptide with the highest antitumor activity from T701 was separated using preparative HPLC system with a C18 column, and prepared into pure powder (≥ 99.5%) by...