Hydrodynamic Chromatography: The Underutilized Size-Based Separation Technique

Hydrodynamic chromatography (HDC) is a size-based liquid chromatographic technique with similar applications as size exclusion chromatography (SEC) and flow field flow fractionation (flow FFF). Separation in HDC is induced within the parabolic flow velocity profile in an open tube or in the interstitial spaces of a packed column, whereby analytes preferentially sample the various streamlines of flow based on size and as a result the larger...