A high‐throughput and simultaneous determination of combretastatin A‐4 phosphate and its metabolites in human plasma using HPLC–MS/MS: Application to a clinical pharmacokinetic study

To investigate the clinical pharmacokinetics of CA4P, a high‐throughput high‐performance liquid chromatography–tandem mass spectrometry assay with an identical positive electrospray ionization (ESI) mode was developed for the simultaneous determination of CA4P, its active metabolite CA4, and CA4 glucuronide in human plasma. CA4P and CA4 were easier to protonate in positive ESI mode, whereas CA4G was reported to produce deprotonated ion in...