A rapid quantitation of cell attachment and spreading based on digital image analysis: Application for cell affinity and compatibility assessment of synthetic polymers

Accurate and rapid quantitation of cell attachment, spreading, and growth on a polymer thin film coated glass cover slide was developed by analyzing the digital images of cells stained with dyes. A biodegradable block copolymer poly(ethylene glycol)- block -poly( l -lactide-co-2-methyl-2-carboxyl-propylene carbonate) [PEG- b -P(LA- co -MCC)] was synthesized as model polymer with poly(L-lactic acid) [PLLA] as a control polymer. Only a small...