A heat-resistant intracellular laccase immobilized via cross-linked enzyme aggregate preparation: Characterization, application in bisphenol A removal and phytotoxicity evaluation

Aureobasidium pullulans laccase was immobilized via cross-linked enzyme aggregate (CLEA) and deployed in bisphenol-A (BPA) removal. The immobilization and BPA removal processes were mathematically modeled. The CLEA-treated BPA was evaluated for phytotoxicity. The optimum conditions for CLEA resulting in the highest immobilization yield were ammonium sulfate (60% w/v), glutaraldehyde (30 mM), pH (4.5), time (6 h) and temperature (45 °C). The CLEA...