Smoking and HIV-1 Infection Promote Retention of CD8+ T Cells in the Airway Mucosa.

Published on Jun 24, 2021in American Journal of Respiratory Cell and Molecular Biology5.373
· DOI :10.1165/RCMB.2021-0168OC
Björn Corleis9
Estimated H-index: 9
(Ragon Institute of MGH, MIT and Harvard),
Josalyn L Cho1
Estimated H-index: 1
(Roy J. and Lucille A. Carver College of Medicine)
+ 10 AuthorsTilo Winkler27
Estimated H-index: 27
(Harvard University)
Smoking and HIV-1 infection are risk factors for COPD, which is among the most common comorbid conditions in people living with HIV-1. HIV-1 infection leads to persistent expansion of CD8+ T cells, and CD8+ T cell-mediated inflammation has been implicated in COPD pathogenesis. In this study, we investigated the effects of HIV-1 infection and smoking on T cell dynamics in patients at risk of COPD. Bronchoalveolar lavage (BAL), endobronchial brushings and blood from HIV-1 infected and uninfected non-smokers and smokers were analyzed by flow cytometry, and lungs were imaged by computed tomography. Chemokines were measured in BAL fluid, and CD8+ T cell chemotaxis in the presence of cigarette smoke extract was assessed in vitro. HIV-1 infection increased CD8+ T cells in the BAL, but this increase was abrogated by smoking. Smokers had reduced BAL levels of the T cell-recruiting chemokines CXCL10 and CCL5, and cigarette smoke extract inhibited CXCL10 and CCL5 production by macrophages and CD8+ T cell transmigration in vitro. In contrast to the BAL, CD8+ T cells in endobronchial brushings were increased in HIV-1 infected smokers, driven by an accumulation of effector memory T cells in the airway mucosa and an increase in tissue resident memory T cells. Mucosal CD8+ T cell numbers inversely correlated with lung aeration, suggesting an association with inflammation and remodeling. HIV-1 infection and smoking lead to retention of CD8+ T cells within the airway mucosa.
📖 Papers frequently viewed together
24 Citations
15 Citations
6 Authors (Martti Laan, ..., Anders Lindén)
24 Citations
Cited By0