Heterogeneous Streptomycin Resistance Level Among Mycobacterium tuberculosis Strains From the Same Transmission Cluster.

Published on Jun 11, 2021in Frontiers in Microbiology5.64
· DOI :10.3389/FMICB.2021.659545
Teresa Carvalho18
Estimated H-index: 18
#1Dipti Shrestha (Hokkaido University)H-Index: 1
#2Bhagwan Maharjan (Kathmandu)H-Index: 10
Last. Yasuhiko Suzuki (Hokkaido University)H-Index: 51
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Abstract Mutation in rpsL (encoding ribosomal protein S12), rrs (encoding 16S ribosomal RNA) and gidB (encoding 7-methylguanosine methyltransferase) are associated with resistance to streptomycin (STR), which is used for the treatment of multi-drug resistant tuberculosis (MDR-TB) in Nepal. The aim of our study is to analyze the correlation between mutations in the target genes and STR-resistance in 197 Mycobacterium tuberculosis (MTB) isolates from Nepal. Mutations in rpsL was harbored by 65.9% ...
#1Giulia Degiacomi (UNIPV: University of Pavia)H-Index: 14
#2Josè Camilla Sammartino (UNIPV: University of Pavia)H-Index: 10
Last. Maria Rosalia Pasca (UNIPV: University of Pavia)H-Index: 32
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Tuberculosis (TB) is one of the major causes of death related to antimicrobial resistance worldwide because of the spread of Mycobacterium tuberculosis multi- and extensively drug resistant (multi-drug resistant (MDR) and extensively drug-resistant (XDR), respectively) clinical isolates. To fight MDR and XDR tuberculosis, three new antitubercular drugs, bedaquiline (BDQ), delamanid, and pretomanid were approved for use in clinical setting. Unfortunately, BDQ quickly acquired two main mechanisms ...
#1Keira A. Cohen (JHUSOM: Johns Hopkins University School of Medicine)H-Index: 13
#2Katharine E. Stott (University of Liverpool)H-Index: 9
Last. Alexander S. PymH-Index: 32
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In 2019, the WHO tuberculosis (TB) treatment guidelines were updated to recommend only limited use of streptomycin, in favor of newer agents or amikacin as the preferred aminoglycoside for drug-resistant Mycobacterium tuberculosis However, the emergence of resistance to newer drugs, such as bedaquiline, has prompted a reanalysis of antitubercular drugs in search of untapped potential. Using 211 clinical isolates of M. tuberculosis from South Africa, we performed phenotypic drug susceptibility te...
#1Mahmudul Islam (CAS: Chinese Academy of Sciences)H-Index: 7
#2Yaoju TanH-Index: 12
Last. Tianyu ZhangH-Index: 25
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Streptomycin (STR) is the first antibiotic used in the treatment of tuberculosis (TB) and the earliest antituberculosis drug with acquired resistance developed by Mycobacterium tuberculosis. The hi...
#1Jeremy Nicolas Carvalho Sousa (IBMC: Instituto de Biologia Molecular e Celular)H-Index: 10
#2Baltazar Cá (IBMC: Instituto de Biologia Molecular e Celular)H-Index: 3
Last. Margarida Saraiva (IBMC: Instituto de Biologia Molecular e Celular)H-Index: 29
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Genetic diversity of Mycobacterium tuberculosis affects immune responses and clinical outcomes of tuberculosis (TB). However, how bacterial diversity orchestrates immune responses to direct distinct TB severities is unknown. Here we study 681 patients with pulmonary TB and show that M. tuberculosis isolates from cases with mild disease consistently induce robust cytokine responses in macrophages across multiple donors. By contrast, bacteria from patients with severe TB do not do so. Secretion of...
We read with great interest the paper by Safi et al. (1) describing frameshifts in glpK ’s homopolymeric tract (HT) of seven cytosines (7C) as a potential cause of antibiotic tolerance. These results have implications for tuberculosis (TB) treatment, but other forces than antibiotic pressure may be responsible for the emergence of glpK mutations (1, 2). We raise the possibilities of selection in vitro, selection in vivo from nonantibiotic host factors, and population bottlenecks in determining t...
#1Hassan Safi (RU: Rutgers University)H-Index: 20
#2David R. Sherman (Seattle Children's)H-Index: 58
Last. David Alland (RU: Rutgers University)H-Index: 28
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We thank Vargas and Farhat (1) for their analyses confirming that reversible glpK frameshifts are common within human Mycobacterium tuberculosis infections. In their thoughtful comments they suggest that additional forces, other than antibiotic pressure, may contribute to the emergence of these mutants. We agree that the mechanisms by which glpK mutants are selected may be complex; however, we question whether their study can resolve if antibiotic pressure in human tuberculosis (TB) increases th...
#1Rhastin A. D. Castro (University of Basel)H-Index: 2
#2Amanda Ross (University of Basel)H-Index: 39
Last. Sebastien Gagneux (University of Basel)H-Index: 76
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: Fluoroquinolones (FQ) form the backbone in experimental treatment regimens against drug-susceptible tuberculosis. However, little is known on whether the genetic variation present in natural populations of Mycobacterium tuberculosis (Mtb) affects the evolution of FQ-resistance (FQ-R). To investigate this question, we used nine genetically distinct drug-susceptible clinical isolates of Mtb and measured their frequency of resistance to the FQ ofloxacin (OFX) in vitro. We found that the Mtb genet...
#1Mercedes C. Becerra (Harvard University)H-Index: 42
#2Chuan-Chin Huang (Brigham and Women's Hospital)H-Index: 18
Last. Paul Farmer (Harvard University)H-Index: 80
view all 14 authors...
Abstract Objective To measure the association between phenotypic drug resistance and the risk of tuberculosis infection and disease among household contacts of patients with pulmonary tuberculosis. Setting 106 district health centers in Lima, Peru between September 2009 and September 2012. Design Prospective cohort study. Participants 10 160 household contacts of 3339 index patients with tuberculosis were classified on the basis of the drug resistance profile of the patient: 6189 were exposed to...
#1Hassan Safi (RU: Rutgers University)H-Index: 20
#2Pooja Gopal (NUS: National University of Singapore)H-Index: 12
Last. David Alland (RU: Rutgers University)H-Index: 28
view all 17 authors...
The length and complexity of tuberculosis (TB) therapy, as well as the propensity of Mycobacterium tuberculosis to develop drug resistance, are major barriers to global TB control efforts. M. tuberculosis is known to have the ability to enter into a drug-tolerant state, which may explain many of these impediments to TB treatment. We have identified a mechanism of genetically encoded but rapidly reversible drug tolerance in M. tuberculosis caused by transient frameshift mutations in a homopolymer...
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