Nanosecond-Resolution Photothermal Dynamic Imaging via MHz Digitization and Match Filtering

Published on Jun 8, 2021
· DOI :10.21203/RS.3.RS-496390/V1
Jiaze Yin , Lu Lan12
Estimated H-index: 12
+ 5 AuthorsJi-Xin Cheng83
Estimated H-index: 83
#1Yi Zhang (BU: Boston University)H-Index: 4
#2Celalettin Yurdakul (BU: Boston University)H-Index: 7
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 8 authors...
We report a confocal interferometric mid-infrared photothermal (MIP) microscope for ultra-sensitive and spatially resolved chemical imaging of individual viruses. The interferometric scattering principle is applied to detect the very weak photothermal signal induced by infrared absorption of chemical bonds. Spectroscopic MIP detection of single vesicular stomatitis viruses (VSVs) and poxviruses is demonstrated. The single virus spectra show high consistency within the same virus type. The domina...
7 CitationsSource
#1Alice Spadea (RMIT: RMIT University)H-Index: 2
#2Joanna Denbigh (University of Salford)H-Index: 5
Last. Peter Gardner (University of Manchester)H-Index: 46
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This paper reports the first use of a novel completely optically based photothermal method (O-PTIR) for obtaining infrared spectra of both fixed and living cells using a quantum cascade laser (QCL) and optical parametric oscillator (OPO) laser as excitation sources, thus enabling all biologically relevant vibrations to be analyzed at submicron spatial resolution. In addition, infrared data acquisition is combined with concomitant Raman spectra from exactly the same excitation location, meaning t...
5 CitationsSource
#1Panagis SamolisH-Index: 3
#2Daniel LangleyH-Index: 9
Last. Michelle Y. SanderH-Index: 13
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Label-free vibrational imaging of biological samples has attracted significant interest due to its integration of structural and chemical information. Vibrational infrared photothermal amplitude and phase signal (VIPPS) imaging provide label-free chemical identification by targeting the characteristic resonances of biological compounds that are present in the mid-infrared fingerprint region (3 µm - 12 µm). High contrast imaging of subcellular features and chemical identification of protein secon...
5 CitationsSource
#1Subhasis Adhikari (LEI: Leiden University)H-Index: 4
#2Patrick Spaeth (LEI: Leiden University)H-Index: 2
Last. Michel Orrit (LEI: Leiden University)H-Index: 61
view all 6 authors...
The photothermal (PT) signal arises from slight changes of the index of refraction in a sample due to absorption of a heating light beam. Refractive index changes are measured with a second probing beam, usually of a different color. In the past two decades, this all-optical detection method has reached the sensitivity of single particles and single molecules, which gave birth to original applications in material science and biology. PT microscopy enables shot-noise-limited detection of individu...
15 CitationsSource
#1Jiabao Xu (University of Oxford)H-Index: 8
#2Xiaojie Li (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 5 authors...
We report rapid and sensitive phenotyping of bacterial response to antibiotic treatment at single-cell resolution by a Raman-integrated optical mid-infrared photothermal (MIP) microscope. The MIP microscope successfully detected biochemical changes of bacteria in specific to the acting mechanism of erythromycin with 1 h incubation. Compared to Raman spectroscopy, MIP spectroscopy showed a much larger signal-to-noise ratio at the fingerprint region at an acquisition speed as fast as 1 s per spect...
4 CitationsSource
#1Ilia M. Pavlovetc (ND: University of Notre Dame)H-Index: 7
#2Michael C. Brennan (UCSC: Catholic University of the Sacred Heart)H-Index: 9
Last. Masaru Kuno (ND: University of Notre Dame)H-Index: 55
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Ion migration represents an intrinsic instability of metal halide perovskite solar cells. Here we show that triple-cation FAxMAyCs1-x-yPbI3 [FA+=(NH2)2CH+, MA+=CH3NH3+] active layers with mixed ort...
11 CitationsSource
#1Tong Ling (Stanford University)H-Index: 11
#2Kevin C. Boyle (Stanford University)H-Index: 5
Last. Daniel Palanker (Stanford University)H-Index: 44
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Neurons undergo nanometer-scale deformations during action potentials, and the underlying mechanism has been actively debated for decades. Previous observations were limited to a single spot or the cell boundary, while movement across the entire neuron during the action potential remained unclear. Here we report full-field imaging of cellular deformations accompanying the action potential in mammalian neuron somas (−1.8 to 1.4 nm) and neurites (−0.7 to 0.9 nm), using high-speed quantitative phas...
20 CitationsSource
#1Ilia M. Pavlovetc (ND: University of Notre Dame)H-Index: 7
#2Eduard A. Podshivaylov (MSU: Moscow State University)H-Index: 2
Last. Masaru Kuno (ND: University of Notre Dame)H-Index: 55
view all 6 authors...
Infrared photothermal heterodyne imaging (IR-PHI) is an ultrasensitive technique for achieving super-resolution, infrared imaging throughout the mid-infrared “fingerprint” spectral region ( 2.5 – ...
14 CitationsSource
#1Miu Tamamitsu (UTokyo: University of Tokyo)H-Index: 10
#2Keiichiro Toda (UTokyo: University of Tokyo)H-Index: 4
Last. Takuro Ideguchi (UTokyo: University of Tokyo)H-Index: 16
view all 9 authors...
Label-free optical imaging is valuable in biology and medicine because of its non-destructive nature. Quantitative phase imaging (QPI) and molecular vibrational imaging (MVI) are the two most successful label-free methods, providing morphological and biochemical information, respectively. These techniques have enabled numerous applications as they have matured over the past few decades; however, their label-free contrasts are inherently complementary and difficult to integrate due to their relia...
15 CitationsSource
#1Wang Yi (University of Oxford)H-Index: 2
#2Jiabao Xu (University of Oxford)H-Index: 8
Last. Chunmiao Zheng (Southern University of Science and Technology)H-Index: 56
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Human intestinal microbiota is important to host health and is associated with various diseases. It is a challenge to identify the functions and metabolic activity of microorganisms at the single-cell level in gut microbial community. In this study, we applied Raman microspectroscopy and deuterium isotope probing (Raman-DIP) to quantitatively measure the metabolic activities of intestinal bacteria from two individuals and analysed lipids and phenylalanine metabolic pathways of functional microor...
17 CitationsSource
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