Nanosecond-Resolution Photothermal Dynamic Imaging via MHz Digitization and Match Filtering

Published on Jun 8, 2021
· DOI :10.21203/RS.3.RS-496390/V1
Jiaze Yin , Lu Lan12
Estimated H-index: 12
+ 5 AuthorsJi-Xin Cheng83
Estimated H-index: 83
Source
Abstract
References47
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This paper reports the first use of a novel completely optically based photothermal method (O-PTIR) for obtaining infrared spectra of both fixed and living cells using a quantum cascade laser (QCL) and optical parametric oscillator (OPO) laser as excitation sources, thus enabling all biologically relevant vibrations to be analyzed at submicron spatial resolution. In addition, infrared data acquisition is combined with concomitant Raman spectra from exactly the same excitation location, meaning t...
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The photothermal (PT) signal arises from slight changes of the index of refraction in a sample due to absorption of a heating light beam. Refractive index changes are measured with a second probing beam, usually of a different color. In the past two decades, this all-optical detection method has reached the sensitivity of single particles and single molecules, which gave birth to original applications in material science and biology. PT microscopy enables shot-noise-limited detection of individu...
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