Female mice exhibit a more sensitive automated squint response to pain induced by CGRP and amylin

Published on May 27, 2021in bioRxiv
· DOI :10.1101/2021.05.26.445893
Brandon J. Rea4
Estimated H-index: 4
(Roy J. and Lucille A. Carver College of Medicine),
Levi P. Sowers10
Estimated H-index: 10
(Roy J. and Lucille A. Carver College of Medicine)
+ 5 AuthorsAndrew F. Russo46
Estimated H-index: 46
(Roy J. and Lucille A. Carver College of Medicine)
We developed an automated squint assay using both black C57BL/6J and white CD1 mice that measured the interpalpebral fissure area between the upper and lower eyelids as an objective quantification of pain. In C57BL/6J mice, we observed a squint response to increasing doses of a migraine trigger, the neuropeptide CGRP, including a significant response in female mice at a dose below detection by the manual grimace scale. Using the automated software, both C57BL/6J and CD1 mice lacked a detectable photic blink response. The CGRP-related peptide amylin induced squinting behavior in female mice, but not males. These data demonstrate that an automated squint assay can be used as an objective, real-time continuous-scale measure of pain that provides higher precision and real-time analysis compared to manual grimace assessments.
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