Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor.

Volume: 22, Issue: 1, Pages: 170 - 170
Published: Jun 3, 2021
Abstract
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with...
Paper Details
Title
Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor.
Published Date
Jun 3, 2021
Volume
22
Issue
1
Pages
170 - 170
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