Tripartite Separation of Glomerular Cell Types and Proteomes from Reporter-Free Mice

Significance Statement Renal blood filtration occurs in a functional unit called the glomerulus. The filtration barrier comprises resident cell types, i.e. , podocytes, mesangial cells, and glomerular endothelial cells. We introduce a glomerular cell isolation protocol that separates these three cell types at a sufficient quantity and purity to allow for detailed protein-biochemical investigations. We demonstrate that the expression of...