Identification of protein interactions of grapevine fanleaf virus RNA-dependent RNA polymerase during infection of Nicotiana benthamiana by affinity purification and tandem mass spectrometry

The RNA-dependent RNA polymerase (1E Pol ) is involved in replication of grapevine fanleaf virus (GFLV, Nepovirus , Secoviridae ) and causes vein clearing symptoms in Nicotiana benthamiana . Information on protein 1E Pol interaction with other viral and host proteins is scarce. To study protein 1E Pol biology, three GFLV infectious clones, i.e. GHu (a symptomatic wild-type strain), GHu-1E K802G (an asymptomatic GHu mutant) and F13 (an...