Nuciferine improves high-fat diet-induced obesity via reducing intestinal permeability by increasing autophagy and remodeling the gut microbiota

Published on Jul 5, 2021in Food & Function4.171
· DOI :10.1039/D1FO00367D
Zhen Shi10
Estimated H-index: 10
(JLU: Jilin University),
Zhiyuan Fang5
Estimated H-index: 5
(JLU: Jilin University)
+ 8 AuthorsGuowen Liu27
Estimated H-index: 27
(JLU: Jilin University)
Nuciferine (NF) has received extensive attention due to its medicinal value in the treatment of metabolic diseases, such as obesity; however, to date, the effects of NF on obesity-related intestinal permeability, autophagy and the gut microbiota have not been investigated. Herein, C57BL/6J mice were fed either a chow or a high-fat diet (HFD) with or without NF for 8 weeks. The results showed that NF supplement reduced weight gain, fat accumulation and intestinal permeability in the HFD mice accompanied by improved autophagy. Subsequently, an in vitro experiment was performed using Caco-2 and HT-29 cells, which showed that NF supplement not only promoted the formation of autophagosomes and autophagolysosomes, but also alleviated LPS-increased intestinal permeability. Importantly, NF supplement protected from LPS-induced paracellular permeability impairment after the administration of autophagy-related gene (Atg) 5 small-interfering RNA (siRNA). These results demonstrate that NF exerts beneficial effects on the intestinal permeability by improving autophagy. Furthermore, we also found that NF supplement lowered the abundance of Butyricimonas and increased the abundance of Akkermansia, an anti-obesity bacterium. Thus, overall, we demonstrated that NF supplement confers reduced intestinal permeability by improving autophagy and alters the composition of the gut microbiota in HFD-fed mice, thereby producing an anti-obesity effect.
📖 Papers frequently viewed together
30 Citations
60 Citations
37 Citations
#1J Grondin (McMaster University)H-Index: 2
#2Huaqing Wang (McMaster University)H-Index: 17
Last. Waliul I. Khan (McMaster University)H-Index: 37
view all 6 authors...
1 CitationsSource
#1Chao Zhang (Xi'an Jiaotong University)H-Index: 8
#2Jianjun Deng (Northwest University (United States))H-Index: 21
Last. Nanping Wang (Xi'an Jiaotong University)H-Index: 43
view all 10 authors...
BACKGROUND AND PURPOSE: Nuciferine, an alkaloid found in Nelumbo nucifera leaves, alleviates dyslipidemia in vivo. However, whether it improves liver injury in diabetic conditions and the underlying mechanism is unclear. The present study aimed to investigate the effects of nuciferine on lipid and glucose metabolism in a murine model of Type 2 diabetes mellitus (T2DM) and to determine the underlying mechanisms of these effects. EXPERIMENTAL APPROACH: A murine model of T2DM was induced by high-fa...
47 CitationsSource
#1Shengnan Shen (UM: University of Macau)H-Index: 7
#2Qiwen Liao (UM: University of Macau)H-Index: 7
Last. Ligen Lin (UM: University of Macau)H-Index: 27
view all 8 authors...
The seeds of Clausena lansium have been used to treat acute and chronic gastro-intestinal disorders. To further explore its therapeutic value on metabolic diseases, a water soluble fraction from ethanolic extract of C. lansium seeds (HP) was evaluated on a high-fat diet (HFD) induced obesity mice model. The mice were fed with HFD for 8 weeks to induce obesity, and then orally administrated with HP for 9 weeks. The results showed HP treatment retarded body weight and fat mass gain of mice under H...
8 CitationsSource
#1Elise Burger (URMC: University of Rochester Medical Center)H-Index: 2
#2Alessandra M. Araujo (URMC: University of Rochester Medical Center)H-Index: 4
Last. Felix Yarovinsky (URMC: University of Rochester Medical Center)H-Index: 34
view all 9 authors...
Summary The protozoan parasite Toxoplasma gondii triggers severe small intestinal immunopathology characterized by IFN-γ- and intestinal microbiota-mediated inflammation, Paneth cell loss, and bacterial dysbiosis. Paneth cells are a prominent secretory epithelial cell type that resides at the base of intestinal crypts and releases antimicrobial peptides. We demonstrate that the microbiota triggers basal Paneth cell-specific autophagy via induction of IFN-γ, a known trigger of autophagy, to maint...
55 CitationsSource
#1Dhirendra P. Singh (Biotechnology Institute)H-Index: 18
#2Pragyanshu Khare (Biotechnology Institute)H-Index: 11
Last. Mahendra Bishnoi (Biotechnology Institute)H-Index: 29
view all 8 authors...
Bacteriostatic properties of a potential anti-obesity agent cinnamaldehyde (CMN) may present untoward effects on the resident gut microbiota. Here, we evaluated whether the combination of Isomalto-oligosaccharides (IMOs) with CMN prevents unwanted effects of CMN on gut microbiota and associated metabolic outcomes in HFD-fed mice. Male Swiss albino mice divided into four groups (n = 10), were fed on normal chow, or HFD (58% fat kcal), HFD + CMN (10 mg kg−1) and HFD + CMN (10 mg kg−1) + IMOs (1 g ...
10 CitationsSource
#1Claire Blanchard (University of Nantes)H-Index: 11
#2François Moreau (University of Nantes)H-Index: 5
Last. Cédric Le May (University of Nantes)H-Index: 21
view all 11 authors...
Background Increased lipopolysaccharide (LPS) translocation due to altered intestinal permeability has been suggested as a mechanism for obesity-associated insulin resistance. The goal of this study was to assess the effect of sleeve gastrectomy (SG) on intestinal barrier permeability in diet-induced obese mice.
20 CitationsSource
The Global Burden of Disease (GBD) study that is now reported in the Journal offers a discouraging reminder that the global obesity epidemic is worsening in most parts of the world and that its implications regarding both physical health and economic health remain ominous.1 The study, in which researchers assembled data from 195 countries to model trends in overweight and obesity and related morbidity and mortality, showed that the prevalence of obesity has more than doubled since 1980 and is no...
141 CitationsSource
#1Sheng WangH-Index: 2
#2Qian Li (Fourth Military Medical University)H-Index: 8
Last. Qibing MeiH-Index: 32
view all 9 authors...
Abstract The saying “An apple a day keeps the doctor away” has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases. It has been well accepted that dysbiosis is the reflection of various chronic diseases. Therefore, this study investigates the effects of apple polysaccharides (AP) on gut dysbiosis. High-fat diet (HFD) fed rats were treated for 14 weeks with AP. The microbiota composition, microbiota-gen...
43 CitationsSource
#2Humberto GiustiH-Index: 10
Last. Rafael Simone SaiaH-Index: 9
view all 5 authors...
Inflammatory mediators have been postulated as elementary inducing factors to the disruption of the intestinal tight junctions (TJ) and consequently, gut permeability and bacterial translocation. Corticosteroids are considered the mainstay in the treatment of septic shock; however, the impact of this therapy on the intestinal epithelial barrier dysfunction during septic shock remains unknown. Our aims were to demonstrate the role of low dexamethasone (DEX) doses in modulation of the inflammatory...
11 CitationsSource
#1Cong Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 5
#2Junkai Yan (SJTU: Shanghai Jiao Tong University)H-Index: 3
Last. Yingwei Chen (SJTU: Shanghai Jiao Tong University)H-Index: 9
view all 7 authors...
Tight junction dysfunction plays a vital role in some chronic inflammatory diseases. Pro-inflammatory cytokines, especially tumor necrosis factor alpha (TNF-α), act as important factors in intestinal epithelial tight junction dysfunction during inflammatory conditions. Autophagy has also been shown to be crucial in tight junction function and claudin-2 expression, but whether autophagy has an effect on the change of claudin-2 expression and tight junction function induced by TNF-α is still unkno...
27 CitationsSource
Cited By0